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Thomas M. Roberts, PhD

Thomas M. Roberts, PhD

Researcher
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(617) 632-3049

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(617) 632-4770

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Biography

Thomas M. Roberts, PhD

Dr. Roberts received his PhD in 1976 from Harvard University, where he also completed a postdoctoral fellowship. In 1981, he joined the DFCI faculty. Dr. Roberts has played a seminal role in discovering the conceptual fabric of signaling downstream from tyrosine kinases.  His lab and that of Lewis Cantley, working together, pioneered research on PI3 kinase.  He has extended his work to stimulate the discovery of clinically useful inhibitors of oncogenic kinases.

Researcher

Physician

Professor of Biological Chemistry and Molecular Pharmacology, Harvard Medical School

Research

    View Dr. Roberts’s Research

    Signaling Mechanisms and Cancer

    Through the process of signal transduction, cells communicate what is happening on their surfaces to the regulatory machinery inside. This process is facilitated by a class of enzymes called kinases, which help activate specific genes in the long strands of DNA in a cell's nucleus. An overactive kinase can lead to an overactive gene and, ultimately, to cancer. Our laboratory is researching the role of these catalysts of cell growth and division and has discovered how several work. Our discoveries have become the basis of new drugs that target the actions of specific kinases. This class of drugs, called kinase inhibitors, offers extraordinary hope for the future of cancer care.

    Research in the laboratory currently focuses on three areas. One is how particular kinases are involved in cancer. For instance, the kinase termed PI3K blocks the orderly process of cell death, called apoptosis. Thus, inhibiting PI3K should lead to tumor cell death. We are also exploring new ways to measure kinase activity in tumors. Every tumor is unique, with its own pattern of activated kinases. Because there are more than 600 different kinases in a given tumor, it is important to find which ones are activated so that we know which ones to inhibit. Finally, our laboratory is developing murine model systems to study kinases in tumors.

    Once kinases have been pinpointed, our laboratory develops the techniques and technology that allow pharmaceutical companies to make new drugs that target them. We supply the company with the reagents necessary to test the effect of drugs on the action of tyrosine kinases. In addition, DFCI scientists have developed the means to make kinases for testing. This collaboration has led to the creation of several new drugs. In particular, the drug Gleevec has been approved by the FDA against chronic myeloid leukemia (CML).  More recently we have been working with Novartis on PI3k inhibitors.  PI3K inhibitors from Novartis and other companies are now entering Phase 2 trials.

    Research Departments

    Publications

      View Dr. Roberts's Publications

      • STING agonism overcomes STAT3-mediated immunosuppression and adaptive resistance to PARP inhibition in ovarian cancer. J Immunother Cancer. 2023 01; 11(1). View in: Pubmed

      • More than one route to render tumors resistant to cGAS/STING activation. Trends Pharmacol Sci. 2023 Feb; 44(2):67-69. View in: Pubmed

      • STING agonism reprograms tumor-associated macrophages and overcomes resistance to PARP inhibition in BRCA1-deficient models of breast cancer. Nat Commun. 2022 05 31; 13(1):3022. View in: Pubmed

      • Blocking PI3K p110ß Attenuates Development of PTEN-Deficient Castration-Resistant Prostate Cancer. Mol Cancer Res. 2022 05 04; 20(5):673-685. View in: Pubmed

      • The Mediator captures CDK7, an attractive transcriptional target in cancer. Cancer Cell. 2021 09 13; 39(9):1184-1186. View in: Pubmed

      • TMTpro-18plex: The Expanded and Complete Set of TMTpro Reagents for Sample Multiplexing. J Proteome Res. 2021 05 07; 20(5):2964-2972. View in: Pubmed

      • The role of the PIK3CA gene in the development and aging of the brain. Sci Rep. 2021 01 11; 11(1):291. View in: Pubmed

      • Divergent Roles of PI3K Isoforms in PTEN-Deficient Glioblastomas. Cell Rep. 2020 09 29; 32(13):108196. View in: Pubmed

      • PIK3CA C-terminal frameshift mutations are novel oncogenic events that sensitize tumors to PI3K-a inhibition. Proc Natl Acad Sci U S A. 2020 09 29; 117(39):24427-24433. View in: Pubmed

      • Inhibition of the transcriptional kinase CDK7 overcomes therapeutic resistance in HER2-positive breast cancers. Oncogene. 2020 01; 39(1):50-63. View in: Pubmed

      • PI3 kinase alpha and delta promote hematopoietic stem cell activation. JCI Insight. 2019 05 23; 5. View in: Pubmed

      • Buparlisib in Patients With Recurrent Glioblastoma Harboring Phosphatidylinositol 3-Kinase Pathway Activation: An Open-Label, Multicenter, Multi-Arm, Phase II Trial. J Clin Oncol. 2019 03 20; 37(9):741-750. View in: Pubmed

      • PARP Inhibition Elicits STING-Dependent Antitumor Immunity in Brca1-Deficient Ovarian Cancer. Cell Rep. 2018 12 11; 25(11):2972-2980.e5. View in: Pubmed

      • Targeted Profiling of RNA Translation. Curr Protoc Mol Biol. 2019 01; 125(1):e71. View in: Pubmed

      • A Conditional Dependency on MELK for the Proliferation of Triple-Negative Breast Cancer Cells. iScience. 2018 Nov 30; 9:149-160. View in: Pubmed

      • Targeted profiling of RNA translation reveals mTOR-4EBP1/2-independent translation regulation of mRNAs encoding ribosomal proteins. Proc Natl Acad Sci U S A. 2018 10 02; 115(40):E9325-E9332. View in: Pubmed

      • Isoform-Selective Phosphatidylinositol 3-Kinase Inhibition in Cancer. J Clin Oncol. 2018 05 01; 36(13):1339-1342. View in: Pubmed

      • CDK4/6 inhibition triggers anti-tumour immunity. Nature. 2017 08 24; 548(7668):471-475. View in: Pubmed

      • CRKL Mediates p110ß-Dependent PI3K Signaling in PTEN-Deficient Cancer Cells. Cell Rep. 2017 07 18; 20(3):549-557. View in: Pubmed

      • PI3K-p110a mediates the oncogenic activity induced by loss of the novel tumor suppressor PI3K-p85a. Proc Natl Acad Sci U S A. 2017 07 03; 114(27):7095-7100. View in: Pubmed

      • Epigenetic regulation of RTK signaling. J Mol Med (Berl). 2017 08; 95(8):791-798. View in: Pubmed

      • The emerging role of PI3K/AKT-mediated epigenetic regulation in cancer. Biochim Biophys Acta Rev Cancer. 2017 Aug; 1868(1):123-131. View in: Pubmed

      • Rac1-mediated membrane raft localization of PI3K/p110ß is required for its activation by GPCRs or PTEN loss. Elife. 2016 10 04; 5. View in: Pubmed

      • NTRK2 activation cooperates with PTEN deficiency in T-ALL through activation of both the PI3K-AKT and JAK-STAT3 pathways. Cell Discov. 2016; 2:16030. View in: Pubmed

      • Transformation by Polyomavirus Middle T Antigen Involves a Unique Bimodal Interaction with the Hippo Effector YAP. J Virol. 2016 08 15; 90(16):7032-7045. View in: Pubmed

      • Combined inhibition of PI3K and PARP is effective in the treatment of ovarian cancer cells with wild-type PIK3CA genes. Gynecol Oncol. 2016 09; 142(3):548-56. View in: Pubmed

      • Tyrosine receptor kinase B is a drug target in astrocytomas. Neuro Oncol. 2017 01; 19(1):22-30. View in: Pubmed

      • PI3K/AKT Signaling Regulates H3K4 Methylation in Breast Cancer. Cell Rep. 2016 06 21; 15(12):2692-704. View in: Pubmed

      • Combination inhibition of PI3K and mTORC1 yields durable remissions in mice bearing orthotopic patient-derived xenografts of HER2-positive breast cancer brain metastases. Nat Med. 2016 07; 22(7):723-6. View in: Pubmed

      • Delivery strategies and potential targets for siRNA in major cancer types. Adv Drug Deliv Rev. 2016 09 01; 104:2-15. View in: Pubmed

      • Effective use of PI3K inhibitor BKM120 and PARP inhibitor Olaparib to treat PIK3CA mutant ovarian cancer. Oncotarget. 2016 Mar 15; 7(11):13153-66. View in: Pubmed

      • Corrigendum: Essential roles of PI(3)K-p110ß in cell growth, metabolism and tumorigenesis. Nature. 2016 05 12; 533(7602):278. View in: Pubmed

      • PI3K-p110a mediates resistance to HER2-targeted therapy in HER2+, PTEN-deficient breast cancers. Oncogene. 2016 07 07; 35(27):3607-12. View in: Pubmed

      • A PI3K p110ß-Rac signalling loop mediates Pten-loss-induced perturbation of haematopoiesis and leukaemogenesis. Nat Commun. 2015 Oct 07; 6:8501. View in: Pubmed

      • Correction for Hwang et al., Polyomavirus small T antigen interacts with yes-associated protein to regulate cell survival and differentiation. J Virol. 2015 Jul; 89(13):6971. View in: Pubmed

      • Papillomavirus E7 oncoproteins share functions with polyomavirus small T antigens. J Virol. 2015 Mar; 89(5):2857-65. View in: Pubmed

      • The mTORC1/S6K1 pathway regulates glutamine metabolism through the eIF4B-dependent control of c-Myc translation. Curr Biol. 2014 Oct 06; 24(19):2274-80. View in: Pubmed

      • Polyomavirus small T antigen interacts with yes-associated protein to regulate cell survival and differentiation. J Virol. 2014 Oct; 88(20):12055-64. View in: Pubmed

      • Polyoma small T antigen triggers cell death via mitotic catastrophe. Oncogene. 2015 May 07; 34(19):2483-92. View in: Pubmed

      • The phosphatidylinositol 3-kinase (PI3K) isoform dependence of tumor formation is determined by the genetic mode of PI3K pathway activation rather than by tissue type. J Virol. 2014 Sep; 88(18):10673-9. View in: Pubmed

      • KRAS and YAP1 converge to regulate EMT and tumor survival. Cell. 2014 Jul 03; 158(1):171-84. View in: Pubmed

      • PI3K isoform dependence of PTEN-deficient tumors can be altered by the genetic context. Proc Natl Acad Sci U S A. 2014 Apr 29; 111(17):6395-400. View in: Pubmed

      • Hematopoiesis and RAS-driven myeloid leukemia differentially require PI3K isoform p110a. J Clin Invest. 2014 Apr; 124(4):1794-809. View in: Pubmed

      • An F876L mutation in androgen receptor confers genetic and phenotypic resistance to MDV3100 (enzalutamide). Cancer Discov. 2013 Sep; 3(9):1030-43. View in: Pubmed

      • Opposing effects of androgen deprivation and targeted therapy on prostate cancer prevention. Cancer Discov. 2013 Jan; 3(1):44-51. View in: Pubmed

      • K-RAS mutant pancreatic tumors show higher sensitivity to MEK than to PI3K inhibition in vivo. PLoS One. 2012; 7(8):e44146. View in: Pubmed

      • Medulloblastoma exome sequencing uncovers subtype-specific somatic mutations. Nature. 2012 Aug 02; 488(7409):106-10. View in: Pubmed

      • The p110a and p110ß isoforms of PI3K play divergent roles in mammary gland development and tumorigenesis. Genes Dev. 2012 Jul 15; 26(14):1573-86. View in: Pubmed

      • Role of major sperm protein (MSP) in the protrusion and retraction of Ascaris sperm. Int Rev Cell Mol Biol. 2012; 297:265-93. View in: Pubmed

      • Reconstitution of amoeboid motility in vitro identifies a motor-independent mechanism for cell body retraction. Curr Biol. 2011 Oct 25; 21(20):1727-31. View in: Pubmed

      • PI3K-targeted therapy can be evaded by gene amplification along the MYC-eukaryotic translation initiation factor 4E (eIF4E) axis. Proc Natl Acad Sci U S A. 2011 Sep 13; 108(37):E699-708. View in: Pubmed

      • Comparisons between murine polyomavirus and Simian virus 40 show significant differences in small T antigen function. J Virol. 2011 Oct; 85(20):10649-58. View in: Pubmed

      • Targeting PI3K signaling as a therapeutic approach for colorectal cancer. Gastroenterology. 2011 Jul; 141(1):50-61. View in: Pubmed

      • Transgenic expression of polyomavirus middle T antigen in the mouse prostate gives rise to carcinoma. J Virol. 2011 Jun; 85(11):5581-92. View in: Pubmed

      • COT drives resistance to RAF inhibition through MAP kinase pathway reactivation. Nature. 2010 Dec 16; 468(7326):968-72. View in: Pubmed

      • Histone H4K20/H3K9 demethylase PHF8 regulates zebrafish brain and craniofacial development. Nature. 2010 Jul 22; 466(7305):503-7. View in: Pubmed

      • A constitutively activated form of the p110beta isoform of PI3-kinase induces prostatic intraepithelial neoplasia in mice. Proc Natl Acad Sci U S A. 2010 Jun 15; 107(24):11002-7. View in: Pubmed

      • Comparing the roles of the p110a and p110ß isoforms of PI3K in signaling and cancer. Curr Top Microbiol Immunol. 2010; 347:55-77. View in: Pubmed

      • Targeting the phosphoinositide 3-kinase pathway in cancer. Nat Rev Drug Discov. 2009 Aug; 8(8):627-44. View in: Pubmed

      • Dephosphorylation of major sperm protein (MSP) fiber protein 3 by protein phosphatase 2A during cell body retraction in the MSP-based amoeboid motility of Ascaris sperm. Mol Biol Cell. 2009 Jul; 20(14):3200-8. View in: Pubmed

      • Should individual PI3 kinase isoforms be targeted in cancer? Curr Opin Cell Biol. 2009 Apr; 21(2):199-208. View in: Pubmed

      • Lessons from polyoma middle T antigen on signaling and transformation: A DNA tumor virus contribution to the war on cancer. Virology. 2009 Feb 20; 384(2):304-16. View in: Pubmed

      • Simian virus 40 large T antigen disrupts genome integrity and activates a DNA damage response via Bub1 binding. J Virol. 2009 Jan; 83(1):117-27. View in: Pubmed

      • A non-canonical function of zebrafish telomerase reverse transcriptase is required for developmental hematopoiesis. PLoS One. 2008; 3(10):e3364. View in: Pubmed

      • The identification of zebrafish mutants showing alterations in senescence-associated biomarkers. PLoS Genet. 2008 Aug 15; 4(8):e1000152. View in: Pubmed

      • Essential roles of PI(3)K-p110beta in cell growth, metabolism and tumorigenesis. Nature. 2008 Aug 07; 454(7205):776-9. View in: Pubmed

      • The role of filament-packing dynamics in powering amoeboid cell motility. Proc Natl Acad Sci U S A. 2008 Apr 08; 105(14):5390-5. View in: Pubmed

      • Protein phosphatase 2A regulates life and death decisions via Akt in a context-dependent manner. Proc Natl Acad Sci U S A. 2007 Nov 27; 104(48):19011-6. View in: Pubmed

      • A histone H3 lysine 27 demethylase regulates animal posterior development. Nature. 2007 Oct 11; 449(7163):689-94. View in: Pubmed

      • Integrative genomic approaches identify IKBKE as a breast cancer oncogene. Cell. 2007 Jun 15; 129(6):1065-79. View in: Pubmed

      • Bub1: escapades in a cellular world. Cell Cycle. 2007 Jul 15; 6(14):1699-704. View in: Pubmed

      • Surveillance mechanism linking Bub1 loss to the p53 pathway. Proc Natl Acad Sci U S A. 2007 May 15; 104(20):8334-9. View in: Pubmed

      • The p110alpha isoform of phosphatidylinositol 3-kinase is essential for polyomavirus middle T antigen-mediated transformation. J Virol. 2007 Jul; 81(13):7069-76. View in: Pubmed

      • Reconstitution in vitro of MSP-based filopodium extension in nematode sperm. Cell Motil Cytoskeleton. 2007 Apr; 64(4):235-47. View in: Pubmed

      • A Ser/Thr kinase required for membrane-associated assembly of the major sperm protein motility apparatus in the amoeboid sperm of Ascaris. Mol Biol Cell. 2007 May; 18(5):1816-25. View in: Pubmed

      • The X-linked mental retardation gene SMCX/JARID1C defines a family of histone H3 lysine 4 demethylases. Cell. 2007 Mar 23; 128(6):1077-88. View in: Pubmed

      • PI3 kinases in cancer: from oncogene artifact to leading cancer target. Sci STKE. 2006 Dec 12; 2006(365):pe52. View in: Pubmed

      • The p110alpha isoform of PI3K is essential for proper growth factor signaling and oncogenic transformation. Proc Natl Acad Sci U S A. 2006 Oct 31; 103(44):16296-300. View in: Pubmed

      • PP2A regulates BCL-2 phosphorylation and proteasome-mediated degradation at the endoplasmic reticulum. J Biol Chem. 2006 Aug 11; 281(32):23003-12. View in: Pubmed

      • Human tumor mutants in the p110alpha subunit of PI3K. Cell Cycle. 2006 Apr; 5(7):675-7. View in: Pubmed

      • Chemical modulation of receptor signaling inhibits regenerative angiogenesis in adult zebrafish. Nat Chem Biol. 2006 May; 2(5):265-73. View in: Pubmed

      • The oncogenic properties of mutant p110alpha and p110beta phosphatidylinositol 3-kinases in human mammary epithelial cells. Proc Natl Acad Sci U S A. 2005 Dec 20; 102(51):18443-8. View in: Pubmed

      • Membrane insertion by anthrax protective antigen in cultured cells. Mol Cell Biol. 2005 Jul; 25(13):5492-8. View in: Pubmed

      • A genetic screen for candidate tumor suppressors identifies REST. Cell. 2005 Jun 17; 121(6):837-48. View in: Pubmed

      • Structure of MFP2 and its function in enhancing MSP polymerization in Ascaris sperm amoeboid motility. J Mol Biol. 2005 Apr 01; 347(3):583-95. View in: Pubmed

      • Preparing to move: assembly of the MSP amoeboid motility apparatus during spermiogenesis in Ascaris. Cell Motil Cytoskeleton. 2005 Apr; 60(4):191-9. View in: Pubmed

      • Major sperm protein. Curr Biol. 2005 Mar 08; 15(5):R153. View in: Pubmed

      • Cytoskeleton dynamics powers nematode sperm motility. Adv Protein Chem. 2005; 71:383-99. View in: Pubmed

      • Comment on "observation of optical precursors in water". Phys Rev Lett. 2004 Dec 31; 93(26 Pt 1):269401. View in: Pubmed

      • Androgen-induced differentiation and tumorigenicity of human prostate epithelial cells. Cancer Res. 2004 Dec 15; 64(24):8867-75. View in: Pubmed

      • The serine/threonine kinase cyclin G-associated kinase regulates epidermal growth factor receptor signaling. Proc Natl Acad Sci U S A. 2004 Jul 13; 101(28):10296-301. View in: Pubmed

      • Functional genetics and experimental models of human cancer. Trends Mol Med. 2004 Jul; 10(7):344-50. View in: Pubmed

      • Simian virus 40 large T antigen targets the spindle assembly checkpoint protein Bub1. Proc Natl Acad Sci U S A. 2004 Jan 27; 101(4):947-52. View in: Pubmed

      • A chemical genetic screen identifies inhibitors of regulated nuclear export of a Forkhead transcription factor in PTEN-deficient tumor cells. Cancer Cell. 2003 Dec; 4(6):463-76. View in: Pubmed

      • Retraction in amoeboid cell motility powered by cytoskeletal dynamics. Science. 2003 Nov 21; 302(5649):1405-7. View in: Pubmed

      • Dissection of the Ascaris sperm motility machinery identifies key proteins involved in major sperm protein-based amoeboid locomotion. Mol Biol Cell. 2003 Dec; 14(12):5082-8. View in: Pubmed

      • A new class of mutations reveals a novel function for the original phosphatidylinositol 3-kinase binding site. Proc Natl Acad Sci U S A. 2003 Aug 05; 100(16):9434-9. View in: Pubmed

      • A 48 kDa integral membrane phosphoprotein orchestrates the cytoskeletal dynamics that generate amoeboid cell motility in Ascaris sperm. J Cell Sci. 2003 Jul 01; 116(Pt 13):2655-63. View in: Pubmed

      • Human mammary epithelial cell transformation through the activation of phosphatidylinositol 3-kinase. Cancer Cell. 2003 May; 3(5):483-95. View in: Pubmed

      • 2.6 A resolution crystal structure of helices of the motile major sperm protein (MSP) of Caenorhabditis elegans. J Mol Biol. 2002 May 31; 319(2):491-9. View in: Pubmed

      • Dissection of angiogenic signaling in zebrafish using a chemical genetic approach. Cancer Cell. 2002 Apr; 1(3):257-67. View in: Pubmed

      • The Hopscotch Jak kinase requires the Raf pathway to promote blood cell activation and differentiation in Drosophila. Mol Genet Genomics. 2002 Mar; 267(1):57-63. View in: Pubmed

      • DEF-1/ASAP1 is a GTPase-activating protein (GAP) for ARF1 that enhances cell motility through a GAP-dependent mechanism. J Biol Chem. 2002 Mar 08; 277(10):7962-9. View in: Pubmed

      • Induction of p53-independent apoptosis by simian virus 40 small t antigen. J Virol. 2001 Oct; 75(19):9142-55. View in: Pubmed

      • Morphogenesis of prechordal plate and notochord requires intact Eph/ephrin B signaling. Dev Biol. 2001 Jun 15; 234(2):470-82. View in: Pubmed

      • Schwann cell proliferative responses to cAMP and Nf1 are mediated by cyclin D1. J Neurosci. 2001 Feb 15; 21(4):1110-6. View in: Pubmed

      • The activation loop in Lck regulates oncogenic potential by inhibiting basal kinase activity and restricting substrate specificity. Oncogene. 2000 Aug 17; 19(35):3961-70. View in: Pubmed

      • Constitutive cellular expression of PI 3-kinase is distinct from transient expression. Biochem Biophys Res Commun. 2000 Jun 16; 272(3):822-9. View in: Pubmed

      • A developmentally regulated switch directs regenerative growth of Schwann cells through cyclin D1. Neuron. 2000 May; 26(2):405-16. View in: Pubmed

      • Abnormal development of Purkinje cells and lymphocytes in Atm mutant mice. Proc Natl Acad Sci U S A. 2000 Mar 28; 97(7):3336-41. View in: Pubmed

      • pRB-dependent, J domain-independent function of simian virus 40 large T antigen in override of p53 growth suppression. J Virol. 2000 Jan; 74(2):864-74. View in: Pubmed

      • Tangled webs: evidence of cross-talk between c-Raf-1 and Akt. Sci STKE. 1999 Dec 21; 1999(13):PE1. View in: Pubmed

      • Heregulin induces phosphorylation of BRCA1 through phosphatidylinositol 3-Kinase/AKT in breast cancer cells. J Biol Chem. 1999 Nov 05; 274(45):32274-8. View in: Pubmed

      • Platelet-derived growth factor stimulation of monocyte chemoattractant protein-1 gene expression is mediated by transient activation of the phosphoinositide 3-kinase signal transduction pathway. J Biol Chem. 1999 Oct 22; 274(43):31062-7. View in: Pubmed

      • Mouse phosphoinositide 3-kinase p110alpha gene: cloning, structural organization, and localization to chromosome 3 band B. Biochem Biophys Res Commun. 1999 Aug 27; 262(2):438-42. View in: Pubmed

      • Tyrosine phosphorylation of the proto-oncoprotein Raf-1 is regulated by Raf-1 itself and the phosphatase Cdc25A. Mol Cell Biol. 1999 Jul; 19(7):4819-24. View in: Pubmed

      • Regulation of G1 progression by the PTEN tumor suppressor protein is linked to inhibition of the phosphatidylinositol 3-kinase/Akt pathway. Proc Natl Acad Sci U S A. 1999 Mar 02; 96(5):2110-5. View in: Pubmed

      • DEF-1, a novel Src SH3 binding protein that promotes adipogenesis in fibroblastic cell lines. Mol Cell Biol. 1999 Mar; 19(3):2330-7. View in: Pubmed

      • Discovery of estrogen-responsive genes using an improved method which combines subtractive hybridization and PCR. Nucleic Acids Res. 1998 Aug 01; 26(15):3616-8. View in: Pubmed

      • Cell density modulates protein-tyrosine phosphorylation. J Biol Chem. 1998 Feb 06; 273(6):3408-14. View in: Pubmed

      • Inactivation of pRB-related proteins p130 and p107 mediated by the J domain of simian virus 40 large T antigen. Mol Cell Biol. 1997 Sep; 17(9):4979-90. View in: Pubmed

      • DnaJ/hsp40 chaperone domain of SV40 large T antigen promotes efficient viral DNA replication. Genes Dev. 1997 May 01; 11(9):1098-110. View in: Pubmed

      • A PDGF-regulated immediate early gene response initiates neuronal differentiation in ventricular zone progenitor cells. Neuron. 1997 Apr; 18(4):553-62. View in: Pubmed

      • Mutation in the Jak kinase JH2 domain hyperactivates Drosophila and mammalian Jak-Stat pathways. Mol Cell Biol. 1997 Mar; 17(3):1562-71. View in: Pubmed

      • Erythropoietin activates Raf1 by an Shc-independent pathway in CTLL-EPO-R cells. Blood. 1997 Jan 01; 89(1):55-64. View in: Pubmed

      • The cytokine-activated tyrosine kinase JAK2 activates Raf-1 in a p21ras-dependent manner. Proc Natl Acad Sci U S A. 1996 Oct 15; 93(21):11681-6. View in: Pubmed

      • Platelet-derived growth factor-induced formation of tensin and phosphoinositide 3-kinase complexes. J Biol Chem. 1996 Sep 20; 271(38):23452-7. View in: Pubmed

      • Intramolecular interactions of the regulatory domains of the Bcr-Abl kinase reveal a novel control mechanism. Structure. 1996 Sep 15; 4(9):1105-14. View in: Pubmed

      • A strategy for screening anti-tumor drugs utilizing oncogenes encoded in retroviral vectors. Int J Cancer. 1996 Jun 11; 66(6):753-9. View in: Pubmed

      • Downregulation of Lck-mediated signal transduction by tip of herpesvirus saimiri. J Virol. 1995 Dec; 69(12):7814-22. View in: Pubmed

      • Identification of Lck-binding elements in tip of herpesvirus saimiri. J Biol Chem. 1995 Sep 01; 270(35):20660-7. View in: Pubmed

      • Reconstitution of signal transduction from the membrane to the nucleus in a baculovirus expression system: activation of Raf-1 leads to hypermodification of c-jun and c-fos via multiple pathways. Oncogene. 1995 Aug 03; 11(3):427-38. View in: Pubmed

      • Identification of regions in polyomavirus middle T and small t antigens important for association with protein phosphatase 2A. J Virol. 1995 Jun; 69(6):3721-8. View in: Pubmed

      • Association of Polyomavirus middle tumor antigen with phospholipase C-gamma 1. J Biol Chem. 1995 May 26; 270(21):12331-4. View in: Pubmed

      • Direct association of Grb2 with the p85 subunit of phosphatidylinositol 3-kinase. J Biol Chem. 1995 May 26; 270(21):12774-80. View in: Pubmed

      • Rapid regeneration of virus from cells infected with a retroviral vector. Biotechniques. 1994 Dec; 17(6):1102-3, 1106-9. View in: Pubmed

      • Interaction of the protein kinase Raf-1 with 14-3-3 proteins. Science. 1994 Oct 07; 266(5182):126-9. View in: Pubmed

      • Association of polyomavirus middle tumor antigen with 14-3-3 proteins. Science. 1994 Jul 22; 265(5171):535-7. View in: Pubmed

      • Polyoma middle tumor antigen interacts with SHC protein via the NPTY (Asn-Pro-Thr-Tyr) motif in middle tumor antigen. Proc Natl Acad Sci U S A. 1994 Jul 05; 91(14):6344-8. View in: Pubmed

      • Signal transduction pathways involving the Raf proto-oncogene. Cancer Metastasis Rev. 1994 Mar; 13(1):105-16. View in: Pubmed

      • Oncogenes and protein kinases in neuronal growth-factor action. Biochim Biophys Acta. 1993 Aug 23; 1155(2):133-50. View in: Pubmed

      • Raf-1 and p21v-ras cooperate in the activation of mitogen-activated protein kinase. Proc Natl Acad Sci U S A. 1993 Jun 15; 90(12):5772-6. View in: Pubmed

      • The cytoplasmic raf oncogene induces a neuronal phenotype in PC12 cells: a potential role for cellular raf kinases in neuronal growth factor signal transduction. Proc Natl Acad Sci U S A. 1993 Jun 01; 90(11):5016-20. View in: Pubmed

      • The conserved lysine of the catalytic domain of protein kinases is actively involved in the phosphotransfer reaction and not required for anchoring ATP. Proc Natl Acad Sci U S A. 1993 Jan 15; 90(2):442-6. View in: Pubmed

      • Selective association between MHC class I-restricted T cell receptors, CDS, and activated tyrosine kinases on thymocytes undergoing positive selection. J Immunol. 1992 Nov 15; 149(10):3142-9. View in: Pubmed

      • Synthetic phosphopeptide immunogens yield activation-specific antibodies to the c-erbB-2 receptor. Proc Natl Acad Sci U S A. 1992 Nov 01; 89(21):10435-9. View in: Pubmed

      • Regulation of the reverse transcriptase of human immunodeficiency virus type 1 by dNTPs. Proc Natl Acad Sci U S A. 1992 Oct 15; 89(20):9720-4. View in: Pubmed

      • Polyomavirus middle T-antigen NPTY mutants. J Virol. 1992 Oct; 66(10):5770-6. View in: Pubmed

      • Tyrosine kinase triggering in thymocytes undergoing positive selection. Eur J Immunol. 1992 Sep; 22(9):2289-94. View in: Pubmed

      • Both p21ras and pp60v-src are required, but neither alone is sufficient, to activate the Raf-1 kinase. Proc Natl Acad Sci U S A. 1992 Apr 01; 89(7):2922-6. View in: Pubmed

      • ras mediates nerve growth factor receptor modulation of three signal-transducing protein kinases: MAP kinase, Raf-1, and RSK. Cell. 1992 Mar 20; 68(6):1041-50. View in: Pubmed

      • Extracellular calcium mimics the actions of platelet-derived growth factor on mouse fibroblasts. Cell Growth Differ. 1992 Mar; 3(3):157-64. View in: Pubmed

      • Transformation-defective mutants of polyomavirus middle T antigen associate with phosphatidylinositol 3-kinase (PI 3-kinase) but are unable to maintain wild-type levels of PI 3-kinase products in intact cells. J Virol. 1992 Mar; 66(3):1702-8. View in: Pubmed

      • The third subunit of protein phosphatase 2A (PP2A), a 55-kilodalton protein which is apparently substituted for by T antigens in complexes with the 36- and 63-kilodalton PP2A subunits, bears little resemblance to T antigens. J Virol. 1992 Feb; 66(2):886-93. View in: Pubmed

      • Purification and initial characterization of the lymphoid-cell protein-tyrosine kinase p56lck from a baculovirus expression system. Proc Natl Acad Sci U S A. 1991 Jul 15; 88(14):6254-8. View in: Pubmed

      • An Epstein-Barr virus protein associated with cell growth transformation interacts with a tyrosine kinase. J Virol. 1991 Jul; 65(7):3681-92. View in: Pubmed

      • Characterization of an active, non-myristylated, cytoplasmic form of the lymphoid protein tyrosine kinase pp56lck. Int Immunol. 1991 Jul; 3(7):673-82. View in: Pubmed

      • Presence of an SH2 domain in the actin-binding protein tensin. Science. 1991 May 03; 252(5006):712-5. View in: Pubmed

      • Granulocyte-macrophage colony-stimulating factor and interleukin-3 induce rapid phosphorylation and activation of the proto-oncogene Raf-1 in a human factor-dependent myeloid cell line. Blood. 1991 Jan 15; 77(2):243-8. View in: Pubmed

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      Dana-Farber Cancer Institute

      450 Brookline Avenue Smith 970A Boston, MA 02215
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      42.3374, -71.1082

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