Dana-Farber Cancer Institute maintains a variety of resources to support the research and educational activities of Dana-Farber faculty. These core facilities, shared resources, and integrative research centers offer research services to the Boston research community, including academic institutions and industry.
The facility provides services and resources for investigators looking to accomplish animal research objectives. This includes housing mice and rats, purchasing animals, training researchers regarding proper animal care and use, and monitoring the safety of all personnel with laboratory animal contact in conjunction with Dana-Farber’s Environmental Health and Safety and Occupational Health Services.
Dana-Farber has established an Institutional Care and Use Committee (IACUC) to oversee the Institute’s animal program, facilities, and projects involving the use of animals. The IACUC serves as the approval body for all protocols involving animal research, and assists faculty, students and staff in upholding Dana-Farber’s commitment to providing the finest care and most humane utilization of laboratory animals.
In addition to basic husbandry services, the ARF staff provides technical and veterinary services, mouse breeding management and mouse irradiation.
Director: Barbara Smith, DVM, PhD Associate Director: Heather Manning, MBAPhone: 617-632-6200Website: research.dfci.harvard.edu/arfsite/
The Dana-Farber Biocontainment Core Facility supports researchers whose experiments require a Biosafety Level 2+ (BL2+) containment level. The Biocontainment core adheres to Biosafety Level 3 (BL3) practices. Protective apparel, equipment, and other materials are provided by the core. Two core facilities are available: one is located at the Jimmy Fund building at Dana Farber; the second at The Center for Life Science. Authorized core users may have access to both facilities.
Use of the facility is limited to researchers who receive training in BL3 procedures and comply with authorized user requirements. Training is provided by the Biosafety Officer, Core Manager, and authorized users.
Jimmy Fund 7 (JFB715-725) and Center for Life Science (CLS 1019B)
Core Manager: Eadaoin (Aideen) Mulligan Email: firstname.lastname@example.orgPhone: 617-632-3377
The Biospecimen Repository provides long-term storage of clinical and research material in -80º C and liquid nitrogen freezers. Transportation of samples to and from the repository is provided for an additional fee. Competitive prices are available on a per box or per freezer basis in either segregated or non-segregated environments.
The core provides affordable monthly fees inclusive of operation and maintenance, and costs that are proportional to your storage needs.
Manager: Evangeline Reynolds Phone: 617-582-7441Website: research4.dfci.harvard.edu/issues/DryDock
Biostatistics provides expertise for the planning, conduct, analysis, and reporting of clinical trials, epidemiologic and population based studies, studies in genetic susceptibility of cancer, and experiments in basic research in the biology of cancer. Services also include: advice and consultation in efficient and accurate database design and management of clinical research data; scientific computing for data analysis and scientific graphics; and education in the areas of study design, data collection, computerization, and statistical methods for laboratory, clinical, and population based studies.
Director: Paul Catalano, ScDDirector: Jane Craycroft, MPHPhone: 617-632-3014Website: www.dfhcc.harvard.edu/core-facilities/biostatistics/
The Clinical Research Laboratory (CRL) utilizes protocol-driven standard operating procedures for the processing of pharmacokinetics, pharmacodynamic, and biomarker samples. This includes the processing, storage, and tracking of all research specimens to ensure the successful completion of clinical trials associated with patient treatment. The CRL is dedicated to the highest standards of specimen handling to maintain the integrity of data procurement and analysis for clinical trials.
Manager: Sujata ShahPhone: 617-632-6981
The Confocal and Light Microscopy Core provides state-of-the-art imaging microscopy technology for fixed and live cells and tissues, along with scientific and technical expertise to assist in experimental design and optimal image collection. Imaging techniques available through the core are standard fluorescence, live-cell long term imaging, confocal, TIRF, FRET, and spectral imaging. Available equipment includes a spinning disk confocal microscope, a Leica SP5 laser scanning confocal with a white light laser, and an inverted widefield fluorescence microscope. Training on all equipment, consultation about various imaging techniques, and assistance with image analysis is also available. Core staff are also able to assist with troubleshooting microscope issues in individual research labs.
Director: Lisa Cameron, PhDPhone: 617-582-8824Website: microscopy.dfci.harvard.edu
The Connell and O’Reilly Families Cell Manipulation Core Facility assists researchers in developing new cell-based therapies for cancer. All cell manufacturing procedures are performed in environmentally controlled conditions according to current Good Manufacturing Practices for cell and tissue processing. Services include the processing of hematopoietic stem cells for autologous or allogeneic transplantation, generation of tumor vaccines using genetic or culture mediated modification of tumor cells, and preparation of immune cell populations for adoptive cellular therapy.
Cell processing for patients enrolled in clinical research protocols, including:
Since most cell manipulation procedures are distinct and developmental, the cost of each procedure is determined individually for each protocol. Charges to principal investigators are based on the laboratory costs determined for each protocol.
Director: Jerome Ritz, MDAssistant Medical Director: Grace Kao, MDPhone: 617-632-2251Website: www.dfhcc.harvard.edu/core-facilities/cell-manipulation/
The Flow Cytometry Core Facility provides sterile sorting and analysis of samples. The core houses three high-speed cell sorters with up to 9-color simultaneous detection utilizing multi-laser platforms. Sample populations can be sterile sorted into a variety of tubes or plates. Single cells can also be sterile sorted into individual wells for culturing. Additionally, the cell sorters can sort up to four populations simultaneously. For sample analysis, the lab houses six tabletop analyzers ranging from very simple 3-color detection systems to a high-end instrument with up to 12-color detection utilizing three lasers. It also has a 96-well plate loader for analyzing samples directly from plates. Staff assist researchers with their sorting and analysis needs, including data acquisition, analysis and interpretation, and experiment design, implementation, and troubleshooting. Training classes are offered for researchers who want to independently run a core flow cytometer.
Director: Wayne Green, PhDEmail: email@example.comPhone: 617-632-3179Website: flowlab.dfci.harvard.edu
The Health Communication Core provides professional creative services to researchers and clinicians from Dana-Farber/Harvard Cancer Center, Dana-Farber Cancer Institute, and other medical and research institutions. The core provides everything from writing, editing, and graphic design to web site development, high-end print production, and audience research. Evidence-based practices with creative expertise are combined to develop materials and communication strategies that help researchers successfully reach their audience.
The core provides creative expertise in the areas of:
Director: Vish Viswanath, PhDCo-Director: Karen Emmons, PhDAssistant Director: Catherine ColemanPhone: 617-632-5078Email: firstname.lastname@example.orgWebsite: www.healthcommcore.org
The Medical Arts Facility Core offers computer and custom graphics and photography services, including single prints, lamination, full posters and titles, brochures, flyers, plasma ads, and special events photography. The core also offers Adobe Photoshop training.
Manager: Minou ModabberPhone: 617-632-3198
The Microarray Core provides services for genome-wide analysis of gene expression, nucleotide variation, copy number variation, and chromatin protein binding sites. The core platforms include microarrays and “next generation” sequencing. Consultation on experimental design and assistance with data analysis is also available.
The Microarray Core Facility provides researchers with array-based services, including gene expression profiling on 3' arrays, Exon 1.0 ST arrays, and on Gene 1.0 ST arrays; genotyping and comparative genomic hybridization both on single nucleotide polymorphism (SNP) arrays, and Chromatin ImmunoPrecipitation localization (ChIP-on-Chip) on tiling arrays. All services use oligonucleotide microarrays. Next Generation Sequencing (NGS) services include DNA-based and RNA-based applications. Biostatistical support to assist in analyzing the data is available.
The laboratory analyses begin with RNA or DNA, provided by investigators, and produce scan data (microarrays) or sequence data (NGS) along with quality control data. These data are available via password-protected accounts accessible through this website.
Biostatistical support is available at the facility to educate investigators about approaches and tools for microarray data analysis. Assistance with concepts and software related to image analysis, statistical methods, clustering, supervised learning and other aspects of microarray data analysis is offered.
Director: Paul MorrisonPhone: 617-632-3082Financial Coordinator: Geraldine Kassirer, MPHPhone: 617-632-2294 Website: chip.dfci.harvard.edu/
The Molecular Biology Core Facilities offer a full suite of molecular biology services, including automated DNA sequencing, genotyping, peptide synthesis, protein sequencing, amino acid analysis, BIA core protein ligand assays, and mass spectrometry. DNA sequencing and genotyping services include synthesis and tracking of oligonucleotides, contig building, and publication to appropriate databases. Various mass spectrometry services are also available, including LTQ Orbitrap and 4800 TOF/TOF.
Director: Paul MorrisonPhone: 617-632-3082Website: mbcf.dfci.harvard.edu
The Molecular Diagnostics Laboratory provides microRNA expression profiling, human cell line identity verification, mutation detection for clinical research studies, and specimen processing for clinical research studies. Consultation on experimental design and assistance with data analysis is also available.
Director: Paul Morrison Phone: 617-632-3082Financial Coordinator: Geraldine Kassirer, MPHPhone: 617-632-2294Website: mdl-web.dfci.harvard.edu
The Monoclonal Antibody Core produces novel monoclonal antibodies for use in basic research, drug discovery, and clinical applications including diagnosis, surrogate markers for disease status, response to therapy, and drug toxicity. Services include cloning and scale-up of immunization antigens to purification of the resulting antibodies. Researchers can choose to have all phases of antibody production conducted by the core or perform particular steps in their own lab.
Core Director: Ed Greenfield, PhDPhone: 617- 632-2209Scientific Advisor: James Decaprio, MDProject Manager: Christina DeOssiePhone: 617-632-6266Website: www.dfhcc.harvard.edu/core-facilities/monoclonal-antibody/;
The RNAi Screening Facility provides investigators with access to RNAi reagents and high-throughput screening technology. Lentiviral-based RNAi constructs against the human and mouse genomes are provided by the RNAi Consortium of the Broad Institute, and can be used to transduce a wide range of target cells, including primary and non-dividing cells. The Facility provides reagents, equipment, and technical expertise to design, optimize, and perform cell-based, arrayed lentiviral RNAi screens. Additionally, lentiviral RNAi constructs against individual genes can be requested.
Request access to iLab SolutionsDirector: William Hahn, MD, PhDCo-Director: Anna Schinzel, PhD Phone: 617-582-7649
The Transgenic Targeting Core offers state-of-the-art instrumentation and staff with scientific and technical expertise in the area of transgenic and gene targeted mouse models. The core assists first time and experienced investigators in the production and characterization of engineered mouse strains. The core provides the essential services, equipment, and reagents, as well as scientific and technical expertise to generate such models. Training is available on both the technical and conceptual levels.
TgT Mouse Core Lab equipment
Director: James HornerPhone: 617-632-6087ES Cell Culture Lab Phone: 617-632-6057
The Tumor Imaging Metrics Core provides standardized, consistent, longitudinal radiological measurements to evaluate therapeutic response for DF/HCC clinical trials. Services include accurate, consistent, and timely analysis of CT, MR, and PET/CT imaging studies. Measurements available include linear (RECIST, WHO, Cheson), volumetric measures for CT or MR scans, and Standardized Uptake Value (SUV) for PET scans. Results of analyses are offered on a password-protected secure web-based report. This core also provides an independent service with verifiable measurement of treatment response for patients enrolled in trials, and serves as a centralized, computerized resource to facilitate efficient internal or external auditing.
Reliable tracking of changes in lesion size or function is essential for clinical trials that use radiological measurements as surrogate endpoints. The Tumor Metrics Core provides a service for managing these images, making measurements in a routine, reliable, and centralized way, and making results available to investigators efficiently.
The Tumor Metrics Core provides four primary services to DF/HCC investigators:
The Tumor Imaging Metrics Core will maintain a database of longitudinal radiological and clinical data that will link to the Quality Assurance for Clinical Trials (QACT) Core to provide an auditable record of quantitative measurements for clinical trials conducted through the DF/HCC.
Director: Gordon Harris, PhDDirector: Mathew Barish, MDDirector: Annick Van den Abbeele, MDPhone: 617-632-2595Website: www.tumormetrics.org
The Center for Cancer Computational Biology (CCCB) provides broad-based support for the analysis and interpretation of 'omic data. The center has two primary elements:
The CCCB provides investigators with the opportunity to work with experienced bioinformatics professionals to address the computational and analytic challenges of their research. The CCCB offers state-of-the-art tools, equipment, and expertise necessary to evaluate the large, complex datasets associated with ‘omic studies and technologies and move beyond the data to a biological interpretation.
A wide range of analytical services for genomic and other 'omic data are available, including: gene expression, copy number variation, ChIP-chip/ ChIP-seq, SNP, and next generation DNA sequencing data. Examples of the type of projects the CCCB can provide assistance with are:
The CCCB and our associated Computational Biology and Functional Genomics Laboratory are composed of a diverse mix of individuals with skills across the scientific/computational spectrum, including biologists, programmers, bioinformaticians, and database and systems administrators. The CCCB maintains an extensive scientific computing infrastructure that includes a high performance compute cluster, large capacity data storage arrays, and high availability clustered Oracle database systems.
Drawing from this varied expertise and infrastructure, the CCCB offers limited support for more general scientific computing needs. Services the CCCB may provide are:
The goal of the CCCB is to leverage member expertise and resources to assist scientists in making the most of their experiments and the data they generate. The analytical and general computing services of the CCCB are available to all researchers at Dana-Farber, as well as external academic and medical institutions, private researchers, and corporations.
Projects at the CCCB follow a collaborative model, and typically begin with an initial consultation where an investigator will meet with an analyst to outline the scientific and technical objectives of the project. Following the initial consultation, our analysts will prepare a detailed analysis plan that includes complete cost and time estimates as well as delivery milestones. Project execution begins once a consensus has been reached on the course of action.
From the beginning of a project to the end, clients will be provided with the following:
All work performed by the CCCB, from analysis plan development to the delivery of final results, undergoes a thorough scientific review to insure the highest standard of quality.
Director: John Quackenbush, PhDEmail: email@example.comPhone: 617-582-9083Website: cccb.dfci.harvard.edu
The Survey and Data Management Core provides efficient, high-quality data collection, management services, and consultation. The core provides expertise and technical support in the areas of development and implementation of data collection protocols from study participants, quality control of data collection processes, and data management. Capabilities include the development and implementation of survey research projects, the development of complex databases, project evaluation, cognitive interviewing, focus groups, in-depth interviews, and other similar processes.
Director: Steven Joffe, MD, MPH Assistant Director: Carol Lowenstein, MBAPhone: 617-582-9063Website: surveycore.org
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