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    Dana H. Gabuzda, MD

    Professor of Neurology, Harvard Medical School
     
     

    About Gabuzda Lab

    HIV molecular biology and pathogenesis 

    The major research interest of our lab is to understand molecular mechanisms of HIV replication and pathogenesis, and their implications for understanding pathogen-host interactions, immune responses, and disease outcomes. Using biological, genetic, biochemical, and systems biology approaches, we study:

    • HIV replication and pathogenesis in the immune system, gut, and central nervous system
    • Mechanisms and pathways underlying differences in host responses to viral infection and antiretroviral therapy
    • Viral diversity and tropism for specific cell types and tissues
    • Functions of the HIV Env, Vif, and Nef proteins
    • High-throughput screening assays to identify small molecules that inhibit Vif interaction with its host cell cofactor APOBEC3G

    Ongoing studies seek to understand the mechanism by which Vif protein overcomes innate antiviral activity of APOBEC3G, a cellular cytidine deaminase that induces G to A hypermutation in newly synthesized viral DNA. Current efforts are focused on understanding the mechanisms by which Vif induces degradation of APOBEC3G through association with a Cullin 5 complex, using high-throughput assays to identify small molecules that inhibit Vif-APOBEC3G interactions. These studies will provide a better understanding of virus-host cell interactions in HIV replication, and will also advance the development of Vif as a target for antiviral therapy.