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Martin E. Hemler, PhD


Researcher


Researcher

  • Professor of Pathology, Harvard Medical School

Contact Information

  • Office Phone Number(617) 632-3410
  • Fax(617) 632-2662

Bio

Dr. Hemler received his PhD in biological chemistry from the University of Michigan in 1978. After postdoctoral research at DFCI from 1979 to 1984, he received a faculty appointment at DFCI and was promoted to professor in 1994. He is exclusively involved in basic laboratory research, focusing on cell surface molecules that participate in cell adhesion, migration, and invasion.

Research



Novel adhesion and palmitoylation mechanisms that regulate the tumor microenvironment



Cell adhesion is a basic process in cell biology, controlling cell growth, death, differentiation, movement, and tissue organization in normal cells, as well as the proliferation and metastasis of tumor cells. The Hemler Lab focuses on the molecular basis for cell adhesion and migration. In particular, they are interested in the structures and functions of heterodimers in the INTEGRIN family, including studies of the mechanisms whereby integrin functions are rapidly turned on and off, and different integrins link to distinct cellular signaling pathways. In addition, they study other cell surface transmembrane proteins that associate with integrins. Recent studies have shown that transmembrane linker proteins, called tetraspanin proteins, allow the membrane proximal extracellular domains of integrins to play key roles in the recruitment of intracellular signaling enzymes such as protein kinase C, and phosphatidylinositol 4-kinase.



Recent studies have focused on how certain integrins may be linked to regulation of matrix metalloproteinase (MMP) production, a key process during cell and tissue remodeling and tumor cell metastasis. Most recently, they have generated mutant mice in which the tetraspanin CD151 gene has been deleted to investigate the role of CD151 during tumor progression.



Sharma C, Hemler ME. Multiple pro-tumor roles for protein acyltransferase DHHC3. Oncoscience. 2017 Nov; 4(11-12):152-153.
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Sharma C, Wang HX, Li Q, Knoblich K, Reisenbichler ES, Richardson AL, Hemler ME. Protein Acyltransferase DHHC3 Regulates Breast Tumor Growth, Oxidative Stress, and Senescence. Cancer Res. 2017 Dec 15; 77(24):6880-6890.
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Wang HX, Hemler ME. Novel impact of EWI-2, CD9, and CD81 on TGF-ß signaling in melanoma. Mol Cell Oncol. 2015 Oct 02; 2(1).
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Wang HX, Sharma C, Knoblich K, Granter SR, Hemler ME. EWI-2 negatively regulates TGF-ß signaling leading to altered melanoma growth and metastasis. Cell Res. 2015 Mar; 25(3):370-85.
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Hemler ME. Tetraspanin proteins promote multiple cancer stages. Nat Rev Cancer. 2014 Jan; 14(1):49-60.
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Hemler ME. Tetraspanin proteins promote multiple cancer stages. Nat Rev Cancer. 2013 Dec 23; 14(1):49-60.
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Knoblich K, Wang HX, Sharma C, Fletcher AL, Turley SJ, Hemler ME. Tetraspanin TSPAN12 regulates tumor growth and metastasis and inhibits ß-catenin degradation. Cell Mol Life Sci. 2014 Apr; 71(7):1305-14.
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Hemler ME, Hoff J, Li Q, Yang XH. Renal disease appears not to affect carcinogenesis in CD151-null mice. Oncogene. 2013 Sep 12; 32(37):4458.
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Deng X, Li Q, Hoff J, Novak M, Yang H, Jin H, Erfani SF, Sharma C, Zhou P, Rabinovitz I, Sonnenberg A, Yi Y, Zhou P, Stipp CS, Kaetzel DM, Hemler ME, Yang XH. Integrin-associated CD151 drives ErbB2-evoked mammary tumor onset and metastasis. Neoplasia. 2012 Aug; 14(8):678-89.
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Li Q, Yang XH, Xu F, Sharma C, Wang HX, Knoblich K, Rabinovitz I, Granter SR, Hemler ME. Tetraspanin CD151 plays a key role in skin squamous cell carcinoma. Oncogene. 2013 Apr 04; 32(14):1772-83.
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Sharma C, Rabinovitz I, Hemler ME. Palmitoylation by DHHC3 is critical for the function, expression, and stability of integrin a6ß4. Cell Mol Life Sci. 2012 Jul; 69(13):2233-44.
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Tsujino K, Takeda Y, Arai T, Shintani Y, Inagaki R, Saiga H, Iwasaki T, Tetsumoto S, Jin Y, Ihara S, Minami T, Suzuki M, Nagatomo I, Inoue K, Kida H, Kijima T, Ito M, Kitaichi M, Inoue Y, Tachibana I, Takeda K, Okumura M, Hemler ME, Kumanogoh A. Tetraspanin CD151 protects against pulmonary fibrosis by maintaining epithelial integrity. Am J Respir Crit Care Med. 2012 Jul 15; 186(2):170-80.
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Malhotra D, Fletcher AL, Astarita J, Lukacs-Kornek V, Tayalia P, Gonzalez SF, Elpek KG, Chang SK, Knoblich K, Hemler ME, Brenner MB, Carroll MC, Mooney DJ, Turley SJ. Transcriptional profiling of stroma from inflamed and resting lymph nodes defines immunological hallmarks. Nat Immunol. 2012 Apr 01; 13(5):499-510.
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Yang XH, Mirchev R, Deng X, Yacono P, Yang HL, Golan DE, Hemler ME. CD151 restricts the a6 integrin diffusion mode. J Cell Sci. 2012 Mar 15; 125(Pt 6):1478-87.
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Wang HX, Kolesnikova TV, Denison C, Gygi SP, Hemler ME. The C-terminal tail of tetraspanin protein CD9 contributes to its function and molecular organization. J Cell Sci. 2011 Aug 15; 124(Pt 16):2702-10.
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Takeda Y, Li Q, Kazarov AR, Epardaud M, Elpek K, Turley SJ, Hemler ME. Diminished metastasis in tetraspanin CD151-knockout mice. Blood. 2011 Jul 14; 118(2):464-72.
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Wang HX, Li Q, Sharma C, Knoblich K, Hemler ME. Tetraspanin protein contributions to cancer. Biochem Soc Trans. 2011 Apr; 39(2):547-52.
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Yang XH, Flores LM, Li Q, Zhou P, Xu F, Krop IE, Hemler ME. Disruption of laminin-integrin-CD151-focal adhesion kinase axis sensitizes breast cancer cells to ErbB2 antagonists. Cancer Res. 2010 Mar 15; 70(6):2256-63.
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Xu D, Sharma C, Hemler ME. Tetraspanin12 regulates ADAM10-dependent cleavage of amyloid precursor protein. FASEB J. 2009 Nov; 23(11):3674-81.
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Lafleur MA, Xu D, Hemler ME. Tetraspanin proteins regulate membrane type-1 matrix metalloproteinase-dependent pericellular proteolysis. Mol Biol Cell. 2009 Apr; 20(7):2030-40.
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Kolesnikova TV, Kazarov AR, Lemieux ME, Lafleur MA, Kesari S, Kung AL, Hemler ME. Glioblastoma inhibition by cell surface immunoglobulin protein EWI-2, in vitro and in vivo. Neoplasia. 2009 Jan; 11(1):77-86, 4p following 86.
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Fiorentino M, Zadra G, Palescandolo E, Fedele G, Bailey D, Fiore C, Nguyen PL, Migita T, Zamponi R, Di Vizio D, Priolo C, Sharma C, Xie W, Hemler ME, Mucci L, Giovannucci E, Finn S, Loda M. Overexpression of fatty acid synthase is associated with palmitoylation of Wnt1 and cytoplasmic stabilization of beta-catenin in prostate cancer. Lab Invest. 2008 Dec; 88(12):1340-8.
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Hemler ME. Targeting of tetraspanin proteins--potential benefits and strategies. Nat Rev Drug Discov. 2008 Sep; 7(9):747-58.
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Sharma C, Yang XH, Hemler ME. DHHC2 affects palmitoylation, stability, and functions of tetraspanins CD9 and CD151. Mol Biol Cell. 2008 Aug; 19(8):3415-25.
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Yang XH, Richardson AL, Torres-Arzayus MI, Zhou P, Sharma C, Kazarov AR, Andzelm MM, Strominger JL, Brown M, Hemler ME. CD151 accelerates breast cancer by regulating alpha 6 integrin function, signaling, and molecular organization. Cancer Res. 2008 May 01; 68(9):3204-13.
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Kovalenko OV, Yang XH, Hemler ME. A novel cysteine cross-linking method reveals a direct association between claudin-1 and tetraspanin CD9. Mol Cell Proteomics. 2007 Nov; 6(11):1855-67.
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Takeda Y, Kazarov AR, Butterfield CE, Hopkins BD, Benjamin LE, Kaipainen A, Hemler ME. Deletion of tetraspanin Cd151 results in decreased pathologic angiogenesis in vivo and in vitro. Blood. 2007 Feb 15; 109(4):1524-32.
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Wunschmann S, Muller HM, Stipp CS, Hemler ME, Stapleton JT. In vitro interaction between hepatitis C virus (HCV) envelope glycoprotein E2 and serum lipoproteins (LPs) results in enhanced cellular binding of both HCV E2 and LPs. J Infect Dis. 2006 Oct 15; 194(8):1058-67.
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Sala-Valdés M, Ursa A, Charrin S, Rubinstein E, Hemler ME, Sánchez-Madrid F, Yáñez-Mó M. EWI-2 and EWI-F link the tetraspanin web to the actin cytoskeleton through their direct association with ezrin-radixin-moesin proteins. J Biol Chem. 2006 Jul 14; 281(28):19665-75.
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Yang XH, Kovalenko OV, Kolesnikova TV, Andzelm MM, Rubinstein E, Strominger JL, Hemler ME. Contrasting effects of EWI proteins, integrins, and protein palmitoylation on cell surface CD9 organization. J Biol Chem. 2006 May 05; 281(18):12976-85.
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Hemler ME. Tetraspanin functions and associated microdomains. Nat Rev Mol Cell Biol. 2005 Oct; 6(10):801-11.
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Kovalenko OV, Metcalf DG, DeGrado WF, Hemler ME. Structural organization and interactions of transmembrane domains in tetraspanin proteins. BMC Struct Biol. 2005 Jun 28; 5:11.
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Xu D, Hemler ME. Metabolic activation-related CD147-CD98 complex. Mol Cell Proteomics. 2005 Aug; 4(8):1061-71.
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Yang X, Kovalenko OV, Tang W, Claas C, Stipp CS, Hemler ME. Palmitoylation supports assembly and function of integrin-tetraspanin complexes. J Cell Biol. 2004 Dec 20; 167(6):1231-40.
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Tang W, Chang SB, Hemler ME. Links between CD147 function, glycosylation, and caveolin-1. Mol Biol Cell. 2004 Sep; 15(9):4043-50.
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Little KD, Hemler ME, Stipp CS. Dynamic regulation of a GPCR-tetraspanin-G protein complex on intact cells: central role of CD81 in facilitating GPR56-Galpha q/11 association. Mol Biol Cell. 2004 May; 15(5):2375-87.
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Kovalenko OV, Yang X, Kolesnikova TV, Hemler ME. Evidence for specific tetraspanin homodimers: inhibition of palmitoylation makes cysteine residues available for cross-linking. Biochem J. 2004 Jan 15; 377(Pt 2):407-17.
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Tang W, Hemler ME. Caveolin-1 regulates matrix metalloproteinases-1 induction and CD147/EMMPRIN cell surface clustering. J Biol Chem. 2004 Mar 19; 279(12):11112-8.
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Kolesnikova TV, Stipp CS, Rao RM, Lane WS, Luscinskas FW, Hemler ME. EWI-2 modulates lymphocyte integrin alpha4beta1 functions. Blood. 2004 Apr 15; 103(8):3013-9.
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Stipp CS, Kolesnikova TV, Hemler ME. EWI-2 regulates alpha3beta1 integrin-dependent cell functions on laminin-5. J Cell Biol. 2003 Dec 08; 163(5):1167-77.
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Lammerding J, Kazarov AR, Huang H, Lee RT, Hemler ME. Tetraspanin CD151 regulates alpha6beta1 integrin adhesion strengthening. Proc Natl Acad Sci U S A. 2003 Jun 24; 100(13):7616-21.
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Stipp CS, Kolesnikova TV, Hemler ME. Functional domains in tetraspanin proteins. Trends Biochem Sci. 2003 Feb; 28(2):106-12.
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Hemler ME. Tetraspanin proteins mediate cellular penetration, invasion, and fusion events and define a novel type of membrane microdomain. Annu Rev Cell Dev Biol. 2003; 19:397-422.
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Kazarov AR, Yang X, Stipp CS, Sehgal B, Hemler ME. An extracellular site on tetraspanin CD151 determines alpha 3 and alpha 6 integrin-dependent cellular morphology. J Cell Biol. 2002 Sep 30; 158(7):1299-309.
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Yang X, Claas C, Kraeft SK, Chen LB, Wang Z, Kreidberg JA, Hemler ME. Palmitoylation of tetraspanin proteins: modulation of CD151 lateral interactions, subcellular distribution, and integrin-dependent cell morphology. Mol Biol Cell. 2002 Mar; 13(3):767-81.
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Zhang XA, Kazarov AR, Yang X, Bontrager AL, Stipp CS, Hemler ME. Function of the tetraspanin CD151-alpha6beta1 integrin complex during cellular morphogenesis. Mol Biol Cell. 2002 Jan; 13(1):1-11.
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Hemler ME. Specific tetraspanin functions. J Cell Biol. 2001 Dec 24; 155(7):1103-7.
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Hemler ME. Retooling of the beta 4 integrin in tumor cells--ligands lost and kinase gained. Dev Cell. 2001 Dec; 1(6):728-30.
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Kolesnikova TV, Mannion BA, Berditchevski F, Hemler ME. Beta1 integrins show specific association with CD98 protein in low density membranes. BMC Biochem. 2001; 2:10.
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Stipp CS, Kolesnikova TV, Hemler ME. EWI-2 is a major CD9 and CD81 partner and member of a novel Ig protein subfamily. J Biol Chem. 2001 Nov 02; 276(44):40545-54.
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Zhang XA, Bontrager AL, Hemler ME. Transmembrane-4 superfamily proteins associate with activated protein kinase C (PKC) and link PKC to specific beta(1) integrins. J Biol Chem. 2001 Jul 06; 276(27):25005-13.
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Sun J, Hemler ME. Regulation of MMP-1 and MMP-2 production through CD147/extracellular matrix metalloproteinase inducer interactions. Cancer Res. 2001 Mar 01; 61(5):2276-81.
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Zhang XA, Bontrager AL, Stipp CS, Kraeft SK, Bazzoni G, Chen LB, Hemler ME. Phosphorylation of a conserved integrin alpha 3 QPSXXE motif regulates signaling, motility, and cytoskeletal engagement. Mol Biol Cell. 2001 Feb; 12(2):351-65.
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Claas C, Stipp CS, Hemler ME. Evaluation of prototype transmembrane 4 superfamily protein complexes and their relation to lipid rafts. J Biol Chem. 2001 Mar 16; 276(11):7974-84.
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Stipp CS, Orlicky D, Hemler ME. FPRP, a major, highly stoichiometric, highly specific CD81- and CD9-associated protein. J Biol Chem. 2001 Feb 16; 276(7):4853-62.
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Yauch RL, Hemler ME. Specific interactions among transmembrane 4 superfamily (TM4SF) proteins and phosphoinositide 4-kinase. Biochem J. 2000 Nov 01; 351 Pt 3:629-37.
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Hemler ME, Rutishauser U. Cell-to-cell contact and extracellular matrix. Editorial overview Curr Opin Cell Biol. 2000 Oct; 12(5):539-41.
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Stipp CS, Hemler ME. Transmembrane-4-superfamily proteins CD151 and CD81 associate with alpha 3 beta 1 integrin, and selectively contribute to alpha 3 beta 1-dependent neurite outgrowth. J Cell Sci. 2000 Jun; 113 ( Pt 11):1871-82.
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Yauch RL, Kazarov AR, Desai B, Lee RT, Hemler ME. Direct extracellular contact between integrin alpha(3)beta(1) and TM4SF protein CD151. J Biol Chem. 2000 Mar 31; 275(13):9230-8.
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Tachibana I, Hemler ME. Role of transmembrane 4 superfamily (TM4SF) proteins CD9 and CD81 in muscle cell fusion and myotube maintenance. J Cell Biol. 1999 Aug 23; 146(4):893-904.
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Seipel K, Medley QG, Kedersha NL, Zhang XA, O'Brien SP, Serra-Pages C, Hemler ME, Streuli M. Trio amino-terminal guanine nucleotide exchange factor domain expression promotes actin cytoskeleton reorganization, cell migration and anchorage-independent cell growth. J Cell Sci. 1999 Jun; 112 ( Pt 12):1825-34.
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Hemler ME. Dystroglycan versatility. Cell. 1999 May 28; 97(5):543-6.
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Zhang XA, Hemler ME. Interaction of the integrin beta1 cytoplasmic domain with ICAP-1 protein. J Biol Chem. 1999 Jan 01; 274(1):11-9.
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Mannion BA, Kolesnikova TV, Lin SH, Wang S, Thompson NL, Hemler ME. The light chain of CD98 is identified as E16/TA1 protein. J Biol Chem. 1998 Dec 11; 273(50):33127-9.
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Yauch RL, Berditchevski F, Harler MB, Reichner J, Hemler ME. Highly stoichiometric, stable, and specific association of integrin alpha3beta1 with CD151 provides a major link to phosphatidylinositol 4-kinase, and may regulate cell migration. Mol Biol Cell. 1998 Oct; 9(10):2751-65.
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Hemler ME. Integrin associated proteins. Curr Opin Cell Biol. 1998 Oct; 10(5):578-85.
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Crommie D, Hemler ME. Beta1 integrin cytoplasmic domain regulates the constitutive conformation detected by MAb 15/7, but not the ligand-induced conformation. J Cell Biochem. 1998 Oct 01; 71(1):63-73.
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Eble JA, Wucherpfennig KW, Gauthier L, Dersch P, Krukonis E, Isberg RR, Hemler ME. Recombinant soluble human alpha 3 beta 1 integrin: purification, processing, regulation, and specific binding to laminin-5 and invasin in a mutually exclusive manner. Biochemistry. 1998 Aug 04; 37(31):10945-55.
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Bazzoni G, Ma L, Blue ML, Hemler ME. Divalent cations and ligands induce conformational changes that are highly divergent among beta1 integrins. J Biol Chem. 1998 Mar 20; 273(12):6670-8.
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Bazzoni G, Hemler ME. Are changes in integrin affinity and conformation overemphasized? Trends Biochem Sci. 1998 Jan; 23(1):30-4.
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Pujades C, Alon R, Yauch RL, Masumoto A, Burkly LC, Chen C, Springer TA, Lobb RR, Hemler ME. Defining extracellular integrin alpha-chain sites that affect cell adhesion and adhesion strengthening without altering soluble ligand binding. Mol Biol Cell. 1997 Dec; 8(12):2647-57.
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Tachibana I, Bodorova J, Berditchevski F, Zutter MM, Hemler ME. NAG-2, a novel transmembrane-4 superfamily (TM4SF) protein that complexes with integrins and other TM4SF proteins. J Biol Chem. 1997 Nov 14; 272(46):29181-9.
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Berditchevski F, Chang S, Bodorova J, Hemler ME. Generation of monoclonal antibodies to integrin-associated proteins. Evidence that alpha3beta1 complexes with EMMPRIN/basigin/OX47/M6. J Biol Chem. 1997 Nov 14; 272(46):29174-80.
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Yauch RL, Felsenfeld DP, Kraeft SK, Chen LB, Sheetz MP, Hemler ME. Mutational evidence for control of cell adhesion through integrin diffusion/clustering, independent of ligand binding. J Exp Med. 1997 Oct 20; 186(8):1347-55.
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Berditchevski F, Tolias KF, Wong K, Carpenter CL, Hemler ME. A novel link between integrins, transmembrane-4 superfamily proteins (CD63 and CD81), and phosphatidylinositol 4-kinase. J Biol Chem. 1997 Jan 31; 272(5):2595-8.
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Weitzman JB, Pujades C, Hemler ME. Integrin alpha chain cytoplasmic tails regulate "antibody-redirected" cell adhesion, independently of ligand binding. Eur J Immunol. 1997 Jan; 27(1):78-84.
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Mannion BA, Berditchevski F, Kraeft SK, Chen LB, Hemler ME. Transmembrane-4 superfamily proteins CD81 (TAPA-1), CD82, CD63, and CD53 specifically associated with integrin alpha 4 beta 1 (CD49d/CD29). J Immunol. 1996 Sep 01; 157(5):2039-47.
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Yuan Q, Strauch KL, Lobb RR, Hemler ME. Intracellular single-chain antibody inhibits integrin VLA-4 maturation and function. Biochem J. 1996 Sep 01; 318 ( Pt 2):591-6.
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Bazzoni G, Carlesso N, Griffin JD, Hemler ME. Bcr/Abl expression stimulates integrin function in hematopoietic cell lines. J Clin Invest. 1996 Jul 15; 98(2):521-8.
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Weitzman JB, Hemler ME, Brodt P. Reduction of tumorigenicity by alpha 3 integrin in a rhabdomyosarcoma cell line. Cell Adhes Commun. 1996 Jul; 4(1):41-52.
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Hemler ME, Mannion BA, Berditchevski F. Association of TM4SF proteins with integrins: relevance to cancer. Biochim Biophys Acta. 1996 Jun 07; 1287(2-3):67-71.
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Berditchevski F, Zutter MM, Hemler ME. Characterization of novel complexes on the cell surface between integrins and proteins with 4 transmembrane domains (TM4 proteins). Mol Biol Cell. 1996 Feb; 7(2):193-207.
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Pujades C, Teixidó J, Bazzoni G, Hemler ME. Integrin alpha 4 cysteines 278 and 717 modulate VLA-4 ligand binding and also contribute to alpha 4/180 formation. Biochem J. 1996 Feb 01; 313 ( Pt 3):899-908.
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Weitzman JB, Chen A, Hemler ME. Investigation of the role of beta 1 integrins in cell-cell adhesion. J Cell Sci. 1995 Nov; 108 ( Pt 11):3635-44.
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Bazzoni G, Shih DT, Buck CA, Hemler ME. Monoclonal antibody 9EG7 defines a novel beta 1 integrin epitope induced by soluble ligand and manganese, but inhibited by calcium. J Biol Chem. 1995 Oct 27; 270(43):25570-7.
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Berditchevski F, Bazzoni G, Hemler ME. Specific association of CD63 with the VLA-3 and VLA-6 integrins. J Biol Chem. 1995 Jul 28; 270(30):17784-90.
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Schiffer SG, Hemler ME, Lobb RR, Tizard R, Osborn L. Molecular mapping of functional antibody binding sites of alpha 4 integrin. J Biol Chem. 1995 Jun 16; 270(24):14270-3.
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Bergelson JM, Hemler ME. Integrin-ligand binding. Do integrins use a 'MIDAS touch' to grasp an Asp? Curr Biol. 1995 Jun 01; 5(6):615-7.
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Kassner PD, Alon R, Springer TA, Hemler ME. Specialized functional properties of the integrin alpha 4 cytoplasmic domain. Mol Biol Cell. 1995 Jun; 6(6):661-74.
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Alon R, Kassner PD, Carr MW, Finger EB, Hemler ME, Springer TA. The integrin VLA-4 supports tethering and rolling in flow on VCAM-1. J Cell Biol. 1995 Mar; 128(6):1243-53.
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Lee RT, Berditchevski F, Cheng GC, Hemler ME. Integrin-mediated collagen matrix reorganization by cultured human vascular smooth muscle cells. Circ Res. 1995 Feb; 76(2):209-14.
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Hemler ME, Lobb RR. The leukocyte beta 1 integrins. Curr Opin Hematol. 1995 Jan; 2(1):61-7.
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Lobb RR, Hemler ME. The pathophysiologic role of alpha 4 integrins in vivo. J Clin Invest. 1994 Nov; 94(5):1722-8.
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Bergelson JM, St John NF, Kawaguchi S, Pasqualini R, Berdichevsky F, Hemler ME, Finberg RW. The I domain is essential for echovirus 1 interaction with VLA-2. Cell Adhes Commun. 1994 Oct; 2(5):455-64.
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Kawaguchi S, Bergelson JM, Finberg RW, Hemler ME. Integrin alpha 2 cytoplasmic domain deletion effects: loss of adhesive activity parallels ligand-independent recruitment into focal adhesions. Mol Biol Cell. 1994 Sep; 5(9):977-88.
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Kassner PD, Kawaguchi S, Hemler ME. Minimum alpha chain cytoplasmic tail sequence needed to support integrin-mediated adhesion. J Biol Chem. 1994 Aug 05; 269(31):19859-67.
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Potts AJ, Croall DE, Hemler ME. Proteolytic cleavage of the integrin beta 4 subunit. Exp Cell Res. 1994 May; 212(1):2-9.
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Pasqualini R, Hemler ME. Contrasting roles for integrin beta 1 and beta 5 cytoplasmic domains in subcellular localization, cell proliferation, and cell migration. J Cell Biol. 1994 Apr; 125(2):447-60.
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Masumoto A, Hemler ME. Mutation of putative divalent cation sites in the alpha 4 subunit of the integrin VLA-4: distinct effects on adhesion to CS1/fibronectin, VCAM-1, and invasin. J Cell Biol. 1993 Oct; 123(1):245-53.
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Kawaguchi S, Hemler ME. Role of the alpha subunit cytoplasmic domain in regulation of adhesive activity mediated by the integrin VLA-2. J Biol Chem. 1993 Aug 05; 268(22):16279-85.
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Mihich E, Hemler ME. Adhesion molecules: cellular recognition mechanisms. The Pezcoller Foundation Symposia, Rovereto, June 24-26, 1992. Ann Oncol. 1993 Aug; 4(7):545-7.
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Kassner PD, Hemler ME. Interchangeable alpha chain cytoplasmic domains play a positive role in control of cell adhesion mediated by VLA-4, a beta 1 integrin. J Exp Med. 1993 Aug 01; 178(2):649-60.
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Bergelson JM, Chan BM, Finberg RW, Hemler ME. The integrin VLA-2 binds echovirus 1 and extracellular matrix ligands by different mechanisms. J Clin Invest. 1993 Jul; 92(1):232-9.
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Hemler M, Mihich E. Fourth annual Pezcoller Symposium--adhesion molecules: cellular recognition mechanisms. Cancer Res. 1993 May 01; 53(9):2192-4.
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Pasqualini R, Bodorova J, Ye S, Hemler ME. A study of the structure, function and distribution of beta 5 integrins using novel anti-beta 5 monoclonal antibodies. J Cell Sci. 1993 May; 105 ( Pt 1):101-11.
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Weitzman JB, Pasqualini R, Takada Y, Hemler ME. The function and distinctive regulation of the integrin VLA-3 in cell adhesion, spreading, and homotypic cell aggregation. J Biol Chem. 1993 Apr 25; 268(12):8651-7.
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Parker CM, Pujades C, Brenner MB, Hemler ME. Alpha 4/180, a novel form of the integrin alpha 4 subunit. J Biol Chem. 1993 Apr 05; 268(10):7028-35.
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Masumoto A, Hemler ME. Multiple activation states of VLA-4. Mechanistic differences between adhesion to CS1/fibronectin and to vascular cell adhesion molecule-1. J Biol Chem. 1993 Jan 05; 268(1):228-34.
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Chan BM, Hemler ME. Multiple functional forms of the integrin VLA-2 can be derived from a single alpha 2 cDNA clone: interconversion of forms induced by an anti-beta 1 antibody. J Cell Biol. 1993 Jan; 120(2):537-43.
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Campanero MR, Arroyo AG, Pulido R, Ursa A, de Matías MS, Sánchez-Mateos P, Kassner PD, Chan BM, Hemler ME, Corbí AL, et al. Functional role of alpha 2/beta 1 and alpha 4/beta 1 integrins in leukocyte intercellular adhesion induced through the common beta 1 subunit. Eur J Immunol. 1992 Dec; 22(12):3111-9.
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Teixidó J, Hemler ME, Greenberger JS, Anklesaria P. Role of beta 1 and beta 2 integrins in the adhesion of human CD34hi stem cells to bone marrow stroma. J Clin Invest. 1992 Aug; 90(2):358-67.
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Chan BM, Elices MJ, Murphy E, Hemler ME. Adhesion to vascular cell adhesion molecule 1 and fibronectin. Comparison of alpha 4 beta 1 (VLA-4) and alpha 4 beta 7 on the human B cell line JY. J Biol Chem. 1992 Apr 25; 267(12):8366-70.
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Bergelson JM, Shepley MP, Chan BM, Hemler ME, Finberg RW. Identification of the integrin VLA-2 as a receptor for echovirus 1. Science. 1992 Mar 27; 255(5052):1718-20.
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Chan BM, Kassner PD, Schiro JA, Byers HR, Kupper TS, Hemler ME. Distinct cellular functions mediated by different VLA integrin alpha subunit cytoplasmic domains. Cell. 1992 Mar 20; 68(6):1051-60.
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Teixidó J, Parker CM, Kassner PD, Hemler ME. Functional and structural analysis of VLA-4 integrin alpha 4 subunit cleavage. J Biol Chem. 1992 Jan 25; 267(3):1786-91.
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Hemler ME, Kassner PD, Chan BM. Functional roles for integrin alpha subunit cytoplasmic domains. Cold Spring Harb Symp Quant Biol. 1992; 57:213-20.
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Kassner PD, Teixido J, Chan BM, Parker CM, Hemler ME. Analyses of VLA-4 structure and function. Adv Exp Med Biol. 1992; 323:163-70.
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Neuhaus H, Hu MC, Hemler ME, Takada Y, Holzmann B, Weissman IL. Cloning and expression of cDNAs for the alpha subunit of the murine lymphocyte-Peyer's patch adhesion molecule. J Cell Biol. 1991 Nov; 115(4):1149-58.
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Schiro JA, Chan BM, Roswit WT, Kassner PD, Pentland AP, Hemler ME, Eisen AZ, Kupper TS. Integrin alpha 2 beta 1 (VLA-2) mediates reorganization and contraction of collagen matrices by human cells. Cell. 1991 Oct 18; 67(2):403-10.
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Takada Y, Murphy E, Pil P, Chen C, Ginsberg MH, Hemler ME. Molecular cloning and expression of the cDNA for alpha 3 subunit of human alpha 3 beta 1 (VLA-3), an integrin receptor for fibronectin, laminin, and collagen. J Cell Biol. 1991 Oct; 115(1):257-66.
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Chan BM, Wong JG, Rao A, Hemler ME. T cell receptor-dependent, antigen-specific stimulation of a murine T cell clone induces a transient, VLA protein-mediated binding to extracellular matrix. J Immunol. 1991 Jul 15; 147(2):398-404.
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Pulido R, Elices MJ, Campanero MR, Osborn L, Schiffer S, García-Pardo A, Lobb R, Hemler ME, Sánchez-Madrid F. Functional evidence for three distinct and independently inhibitable adhesion activities mediated by the human integrin VLA-4. Correlation with distinct alpha 4 epitopes. J Biol Chem. 1991 Jun 05; 266(16):10241-5.
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Chan BM, Matsuura N, Takada Y, Zetter BR, Hemler ME. In vitro and in vivo consequences of VLA-2 expression on rhabdomyosarcoma cells. Science. 1991 Mar 29; 251(5001):1600-2.
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Elices MJ, Urry LA, Hemler ME. Receptor functions for the integrin VLA-3: fibronectin, collagen, and laminin binding are differentially influenced by Arg-Gly-Asp peptide and by divalent cations. J Cell Biol. 1991 Jan; 112(1):169-81.
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Hemler ME, Elices MJ, Chan BM, Zetter B, Matsuura N, Takada Y. Multiple ligand binding functions for VLA-2 (alpha 2 beta 1) and VLA-3 (alpha 3 beta 1) in the integrin family. Cell Differ Dev. 1990 Dec 02; 32(3):229-38.
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Ramaswamy H, Hemler ME. Cloning, primary structure and properties of a novel human integrin beta subunit. EMBO J. 1990 May; 9(5):1561-8.
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Hemler ME, Elices MJ, Parker C, Takada Y. Structure of the integrin VLA-4 and its cell-cell and cell-matrix adhesion functions. Immunol Rev. 1990 Apr; 114:45-65.
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Elices MJ, Osborn L, Takada Y, Crouse C, Luhowskyj S, Hemler ME, Lobb RR. VCAM-1 on activated endothelium interacts with the leukocyte integrin VLA-4 at a site distinct from the VLA-4/fibronectin binding site. Cell. 1990 Feb 23; 60(4):577-84.
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Hemler ME. VLA proteins in the integrin family: structures, functions, and their role on leukocytes. Annu Rev Immunol. 1990; 8:365-400.
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Elices MJ, Hemler ME. The human integrin VLA-2 is a collagen receptor on some cells and a collagen/laminin receptor on others. Proc Natl Acad Sci U S A. 1989 Dec; 86(24):9906-10.
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Kennel SJ, Foote LJ, Falcioni R, Sonnenberg A, Stringer CD, Crouse C, Hemler ME. Analysis of the tumor-associated antigen TSP-180. Identity with alpha 6-beta 4 in the integrin superfamily. J Biol Chem. 1989 Sep 15; 264(26):15515-21.
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Bank I, Hemler M, Brenner MB, Cohen D, Levy V, Belko J, Crouse C, Chess L. A novel monoclonal antibody, 1B3.1, binds to a new epitope of the VLA-1 molecule. Cell Immunol. 1989 Sep; 122(2):416-23.
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Takada Y, Hemler ME. The primary structure of the VLA-2/collagen receptor alpha 2 subunit (platelet GPIa): homology to other integrins and the presence of a possible collagen-binding domain. J Cell Biol. 1989 Jul; 109(1):397-407.
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Takada Y, Elices MJ, Crouse C, Hemler ME. The primary structure of the alpha 4 subunit of VLA-4: homology to other integrins and a possible cell-cell adhesion function. EMBO J. 1989 May; 8(5):1361-8.
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Hemler ME, Crouse C, Sonnenberg A. Association of the VLA alpha 6 subunit with a novel protein. A possible alternative to the common VLA beta 1 subunit on certain cell lines. J Biol Chem. 1989 Apr 15; 264(11):6529-35.
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Heino J, Ignotz RA, Hemler ME, Crouse C, Massagué J. Regulation of cell adhesion receptors by transforming growth factor-beta. Concomitant regulation of integrins that share a common beta 1 subunit. J Biol Chem. 1989 Jan 05; 264(1):380-8.
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Takada Y, Wayner EA, Carter WG, Hemler ME. Extracellular matrix receptors, ECMRII and ECMRI, for collagen and fibronectin correspond to VLA-2 and VLA-3 in the VLA family of heterodimers. J Cell Biochem. 1988 Aug; 37(4):385-93.
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Fingerman E, Hemler ME. Regulation of proteins in the VLA cell substrate adhesion family: influence of cell growth conditions on VLA-1, VLA-2, and VLA-3 expression. Exp Cell Res. 1988 Jul; 177(1):132-42.
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Hemler ME, Crouse C, Takada Y, Sonnenberg A. Multiple very late antigen (VLA) heterodimers on platelets. Evidence for distinct VLA-2, VLA-5 (fibronectin receptor), and VLA-6 structures. J Biol Chem. 1988 Jun 05; 263(16):7660-5.
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Hemler ME. Adhesive protein receptors on hematopoietic cells. Immunol Today. 1988 Apr; 9(4):109-13.
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Saltini C, Hemler ME, Crystal RG. T lymphocytes compartmentalized on the epithelial surface of the lower respiratory tract express the very late activation antigen complex VLA-1. Clin Immunol Immunopathol. 1988 Feb; 46(2):221-33.
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Hemler ME, Huang C, Takada Y, Schwarz L, Strominger JL, Clabby ML. Characterization of the cell surface heterodimer VLA-4 and related peptides. J Biol Chem. 1987 Aug 25; 262(24):11478-85.
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Hemler ME, Jacobson JG. Cell matrix adhesion-related proteins VLA-1 and VLA-2: regulation of expression on T cells. J Immunol. 1987 May 01; 138(9):2941-8.
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Takada Y, Strominger JL, Hemler ME. The very late antigen family of heterodimers is part of a superfamily of molecules involved in adhesion and embryogenesis. Proc Natl Acad Sci U S A. 1987 May; 84(10):3239-43.
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Takada Y, Huang C, Hemler ME. Fibronectin receptor structures in the VLA family of heterodimers. Nature. 1987 Apr 9-15; 326(6113):607-9.
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Hemler ME, Huang C, Schwarz L. The VLA protein family. Characterization of five distinct cell surface heterodimers each with a common 130,000 molecular weight beta subunit. J Biol Chem. 1987 Mar 05; 262(7):3300-9.
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Pischel KD, Hemler ME, Huang C, Bluestein HG, Woods VL. Use of the monoclonal antibody 12F1 to characterize the differentiation antigen VLA-2. J Immunol. 1987 Jan 01; 138(1):226-33.
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Hemler ME, Glass D, Coblyn JS, Jacobson JG. Very late activation antigens on rheumatoid synovial fluid T lymphocytes. Association with stages of T cell activation. J Clin Invest. 1986 Sep; 78(3):696-702.
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Hemler ME, Jacobson JG, Strominger JL. Biochemical characterization of VLA-1 and VLA-2. Cell surface heterodimers on activated T cells. J Biol Chem. 1985 Dec 05; 260(28):15246-52.
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Hafler DA, Hemler ME, Christenson L, Williams JM, Shapiro HM, Strom TB, Strominger JL, Weiner HL. Investigation of in vivo activated T cells in multiple sclerosis and inflammatory central nervous system diseases. Clin Immunol Immunopathol. 1985 Nov; 37(2):163-71.
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Hemler ME, Jacobson JG, Brenner MB, Mann D, Strominger JL. VLA-1: a T cell surface antigen which defines a novel late stage of human T cell activation. Eur J Immunol. 1985 May; 15(5):502-8.
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Hemler ME, Sanchez-Madrid F, Flotte TJ, Krensky AM, Burakoff SJ, Bhan AK, Springer TA, Strominger JL. Glycoproteins of 210,000 and 130,000 m.w. on activated T cells: cell distribution and antigenic relation to components on resting cells and T cell lines. J Immunol. 1984 Jun; 132(6):3011-8.
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Peters PM, Kamarck ME, Hemler ME, Strominger JL, Ruddle FH. Genetic and biochemical characterization of human lymphocyte cell surface antigens. The A-1A5 and A-3A4 determinants. J Exp Med. 1984 May 01; 159(5):1441-54.
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Hemler ME, Brenner MB, McLean JM, Strominger JL. Antigenic stimulation regulates the level of expression of interleukin 2 receptor on human T cells. Proc Natl Acad Sci U S A. 1984 Apr; 81(7):2172-5.
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Hemler ME, Malissen B, Rebai N, Liabeuf A, Mawas C, Kourilsky FM, Strominger JL. A 55,000 Mr surface antigen on activated human T lymphocytes defined by a monoclonal antibody. Hum Immunol. 1983 Oct; 8(2):153-65.
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Haynes BF, Harden EA, Telen MJ, Hemler ME, Strominger JL, Palker TJ, Scearce RM, Eisenbarth GS. Differentiation of human T lymphocytes. I. Acquisition of a novel human cell surface protein (p80) during normal intrathymic T cell maturation. J Immunol. 1983 Sep; 131(3):1195-200.
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Hemler ME, Ware CF, Strominger JL. Characterization of a novel differentiation antigen complex recognize by a monoclonal antibody (A-1A5): unique activation-specific molecular forms on stimulated T cells. J Immunol. 1983 Jul; 131(1):334-40.
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Burakoff SJ, Clayberger C, Hemler M, Krensky AM, Reiss CS, Robbins E, Sanchez-Madrid F, Springer TA, Strominger JL, Ware CF. Cytotoxic T cells directed against HLA-DR antigens and their surface proteins. Diagn Immunol. 1983; 1(3):116-9.
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Hemler ME, Strominger JL. Monoclonal antibodies reacting with immunogenic mycoplasma proteins present in human hematopoietic cell lines. J Immunol. 1982 Dec; 129(6):2734-8.
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Peters PG, Kamarck ME, Hemler ME, Strominger JL, Ruddle FH. Genetic and biochemical characterization of a human surface determinant on somatic cell hybrids: the 4F2 antigen. Somatic Cell Genet. 1982 Nov; 8(6):825-34.
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Hemler ME, Strominger JL. Characterization of antigen recognized by the monoclonal antibody (4F2): different molecular forms on human T and B lymphoblastoid cell lines. J Immunol. 1982 Aug; 129(2):623-8.
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Haynes BF, Reisner EG, Hemler ME, Strominger JL, Eisenbarth GS. Description of monoclonal antibody defining an HLA allotypic determinant that includes specificities within the B5 cross-reacting group. Hum Immunol. 1982 Jul; 4(4):273-85.
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Haynes BF, Hemler M, Cotner T, Mann DL, Eisenbarth GS, Strominger JL, Fauci AS. Characterization of a monoclonal antibody (5E9) that defines a human cell surface antigen of cell activation. J Immunol. 1981 Jul; 127(1):347-51.
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Irigoyen OH, Rizzolo PV, Thomas Y, Hemler ME, Shen HH, Friedman SM, Strominger JL, Chess L. Dissection of distinct human immunoregulatory T-cell subsets by a monoclonal antibody recognizing a cell surface antigen with wide tissue distribution. Proc Natl Acad Sci U S A. 1981 May; 78(5):3160-4.
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Haynes BF, Hemler ME, Mann DL, Eisenbarth GS, Shelhamer J, Mostowski HS, Thomas CA, Strominger JL, Fauci AS. Characterization of a monoclonal antibody (4F2) that binds to human monocytes and to a subset of activated lymphocytes. J Immunol. 1981 Apr; 126(4):1409-14.
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Cotner T, Hemler M, Strominger JL. Human T cell proteins recognized by rabbit heteroantisera and monoclonal antibodies. Int J Immunopharmacol. 1981; 3(3):255-68.
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Reiss CS, Hemler ME, Englehard VH, Mier JW, Strominger JL, Burakoff SJ. Development and characterization of allospecific long-term human cytolytic T-cell lines. Proc Natl Acad Sci U S A. 1980 Sep; 77(9):5432-6.
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Hemler ME, Lands WE. Evidence for a peroxide-initiated free radical mechanism of prostaglandin biosynthesis. J Biol Chem. 1980 Jul 10; 255(13):6253-61.
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Haynes BF, Mann DL, Hemler ME, Schroer JA, Shelhamer JH, Eisenbarth GS, Strominger JL, Thomas CA, Mostowski HS, Fauci AS. Characterization of a monoclonal antibody that defines an immunoregulatory T cell subset for immunoglobulin synthesis in humans. Proc Natl Acad Sci U S A. 1980 May; 77(5):2914-8.
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Hemler ME, Lands WE. Protection of cyclooxygenase activity during heme-induced destabilization. Arch Biochem Biophys. 1980 May; 201(2):586-93.
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Hemler ME, Cook HW, Lands WE. Prostaglandin biosynthesis can be triggered by lipid peroxides. Arch Biochem Biophys. 1979 Apr 01; 193(2):340-5.
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Hemler ME, Graff G, Lands WE. Accelerative autoactivation of prostaglandin biosynthesis by PGG2. Biochem Biophys Res Commun. 1978 Dec 29; 85(4):1325-31.
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Hemler ME, Crawford CG, Lands WE. Lipoxygenation activity of purified prostaglandin-forming cyclooxygenase. Biochemistry. 1978 May 02; 17(9):1772-9.
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Dawson MI, Reid M, Hemler ME, Lands WE. Synthesis of prostaglandin synthetase substrate analogues. 2. (8Z,11Z,14Z)-15-Methyl-8,11,14-eicosatrienoic acid. J Med Chem. 1977 Nov; 20(11):1396-400.
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Hemler ME, Lands WE. Biosynthesis of prostaglandins. Lipids. 1977 Jul; 12(7):591-5.
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Hemler M, Lands WE. Purification of the cyclooxygenase that forms prostaglandins. Demonstration of two forms of iron in the holoenzyme. J Biol Chem. 1976 Sep 25; 251(18):5575-9.
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