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Wayne A. Marasco, MD, PhD


Researcher


Researcher

  • Brigham and Women's Hospital, Associate Physician, Division of Infectious Diseases, Department of Medicine
  • Professor of Medicine, Harvard Medical School

Contact Information

  • Office Phone Number617-632-2153
  • Fax617-632-3889

Bio

Dr. Marasco received his PhD in 1980 from the University of Connecticut School of Medicine and postdoctoral training at the University of Michigan Medical School, where he also earned an MD in 1986 and completed training in internal medicine. He received his subspecialty training in infectious diseases at Harvard Medical School, and joined DFCI in 1989. In 2003, he founded the National Foundation of Cancer Research Center for Therapeutic Antibody Engineering to expand the use of human monoclonal antibodies in the treatment of cancer. In 2009, he was listed among 13 top scientists in their field as the 21st century medicine "Pioneeers of Medicine Progress" by US News & World report. He is head of an accomplished research laboratory in the area of cancer and infectious disease immunotherapy.

Recent Awards:

  • "Pioneer of Medical Progress", U.S. News & World Report (among 13 top scientists in their field) 2009

Research

Human monoclonal antibody-based immunotherapy

Our laboratory focuses on the engineering and use of human antibodies in discovery research and disease treatment. We are working in three disease areas: cancer, emerging infectious diseases, and HIV/AIDS – a breadth of investigation made possible by the development and use of state-of-the-art tools in the field of antibody engineering that support our research efforts. More recently, we have been developing humanized mice to support our targeted immunotherapy studies and to investigate the roles of human adult stem cells in regenerative medicine.

We have active research programs in cancer immunotherapy that include the following targets: CXCR4 for breast cancer, G250 (CA IX) for renal cell carcinoma, CCR4 for cutaneous T cell lymphomas (CTCLs), and IGHV1-69 encoded B-cell receptors (BCR) for treatment of an aggressive form of chronic lymphocytic leukemia (CLL). We are also developing immunotherapies that can modulate immune T cell activity to boost natural cancer immunity and response to cancer vaccines. Regulatory T cells (Tregs) exert control by dampening the immune response to prevent host tissue damage, but these cells are also co-opted by cancer cells, which leads to immune evasion. Tregs express high levels of CCR4 and we have demonstrated in vitro and in vivo that anti-CCR4 Mabs can reverse this immune suppression and reverse T cell "exhaustion," thereby restoring host immunity.

We use human monoclonal antibodies in functional and structural studies to understand mechanisms of viral entry; identify common targets that provide broad-spectrum protection; and develop strategies that prevent the viruses from undergoing neutralization escape. Vaccinated/infected humans and humanized mice are the B-cell sources of antibody genes for these studies. We are studying human pathogenic viruses that include influenza A, West Nile Virus, SARS- and MERS-CoVs and HIV/AIDS. As with our cancer studies, we are investigating whether human antibodies that are directed to surface proteins on T-cells or their secreted products can reverse T-cell "exhaustion" which is known to occur in HIV-1/AIDS with the goal of lowering viral loads, prolonging health, and preventing the spread of HIV-1. We are using our humanized mice in the area of anti-microbial immunity to develop broad-spectrum anti-viral vaccines.

Canale AS, Venev SV, Whitfield TW, Caffrey DR, Marasco WA, Schiffer CA, Kowalik TF, Jensen JD, Finberg RW, Zeldovich KB, Wang JP, Bolon DNA. Synonymous Mutations at the Beginning of the Influenza A Virus Hemagglutinin Gene Impact Experimental Fitness. J Mol Biol. 2018 Apr 13; 430(8):1098-1115.
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Magnotti E, Marasco WA. The latest animal models of ovarian cancer for novel drug discovery. Expert Opin Drug Discov. 2018 Mar; 13(3):249-257.
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Avnir Y, Prachanronarong KL, Zhang Z, Hou S, Peterson EC, Sui J, Zayed H, Kurella VB, McGuire AT, Stamatatos L, Hilbert BJ, Bohn MF, Kowalik TF, Jensen JD, Finberg RW, Wang JP, Goodall M, Jefferis R, Zhu Q, Kurt Yilmaz N, Schiffer CA, Marasco WA. Structural Determination of the Broadly Reactive Anti-IGHV1-69 Anti-idiotypic Antibody G6 and Its Idiotope. Cell Rep. 2017 Dec 12; 21(11):3243-3255.
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Pierce CL, Williams TL, Santana WI, Levine M, Chen LM, Cooper HC, Solano MI, Woolfitt AR, Marasco WA, Fang H, Donis RO, Barr JR. Immunocapture isotope dilution mass spectrometry in response to a pandemic influenza threat. Vaccine. 2017 09 05; 35(37):5011-5018.
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Watson CT, Glanville J, Marasco WA. The Individual and Population Genetics of Antibody Immunity. Trends Immunol. 2017 07; 38(7):459-470.
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Cockrell AS, Yount BL, Scobey T, Jensen K, Douglas M, Beall A, Tang XC, Marasco WA, Heise MT, Baric RS. A mouse model for MERS coronavirus-induced acute respiratory distress syndrome. Nat Microbiol. 2016 Nov 28; 2:16226.
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Simmons M, Putnak R, Sun P, Burgess T, Marasco WA. Antibody Prophylaxis Against Dengue Virus 2 Infection in Non-Human Primates. Am J Trop Med Hyg. 2016 Nov 02; 95(5):1148-1156.
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Fu Y, Zhang Z, Sheehan J, Avnir Y, Ridenour C, Sachnik T, Sun J, Hossain MJ, Chen LM, Zhu Q, Donis RO, Marasco WA. A broadly neutralizing anti-influenza antibody reveals ongoing capacity of haemagglutinin-specific memory B cells to evolve. Nat Commun. 2016 Sep 13; 7:12780.
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Suarez ER, Chang de K, Sun J, Sui J, Freeman GJ, Signoretti S, Zhu Q, Marasco WA. Chimeric antigen receptor T cells secreting anti-PD-L1 antibodies more effectively regress renal cell carcinoma in a humanized mouse model. Oncotarget. 2016 Jun 07; 7(23):34341-55.
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Avnir Y, Watson CT, Glanville J, Peterson EC, Tallarico AS, Bennett AS, Qin K, Fu Y, Huang CY, Beigel JH, Breden F, Zhu Q, Marasco WA. Erratum: IGHV1-69 polymorphism modulates anti-influenza antibody repertoires, correlates with IGHV utilization shifts and varies by ethnicity. Sci Rep. 2016 04 29; 6:23876.
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Menachery VD, Yount BL, Debbink K, Agnihothram S, Gralinski LE, Plante JA, Graham RL, Scobey T, Ge XY, Donaldson EF, Randell SH, Lanzavecchia A, Marasco WA, Shi ZL, Baric RS. Corrigendum: A SARS-like cluster of circulating bat coronaviruses shows potential for human emergence. Nat Med. 2016 Apr; 22(4):446.
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Menachery VD, Yount BL, Sims AC, Debbink K, Agnihothram SS, Gralinski LE, Graham RL, Scobey T, Plante JA, Royal SR, Swanstrom J, Sheahan TP, Pickles RJ, Corti D, Randell SH, Lanzavecchia A, Marasco WA, Baric RS. SARS-like WIV1-CoV poised for human emergence. Proc Natl Acad Sci U S A. 2016 Mar 15; 113(11):3048-53.
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Chang DK, Kurella VB, Biswas S, Avnir Y, Sui J, Wang X, Sun J, Wang Y, Panditrao M, Peterson E, Tallarico A, Fernandes S, Goodall M, Zhu Q, Brown JR, Jefferis R, Marasco WA. Humanized mouse G6 anti-idiotypic monoclonal antibody has therapeutic potential against IGHV1-69 germline gene-based B-CLL. MAbs. 2016 May-Jun; 8(4):787-98.
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Avnir Y, Watson CT, Glanville J, Peterson EC, Tallarico AS, Bennett AS, Qin K, Fu Y, Huang CY, Beigel JH, Breden F, Zhu Q, Marasco WA. IGHV1-69 polymorphism modulates anti-influenza antibody repertoires, correlates with IGHV utilization shifts and varies by ethnicity. Sci Rep. 2016 Feb 16; 6:20842.
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Johnson RF, Bagci U, Keith L, Tang X, Mollura DJ, Zeitlin L, Qin J, Huzella L, Bartos CJ, Bohorova N, Bohorov O, Goodman C, Kim DH, Paulty MH, Velasco J, Whaley KJ, Johnson JC, Pettitt J, Ork BL, Solomon J, Oberlander N, Zhu Q, Sun J, Holbrook MR, Olinger GG, Baric RS, Hensley LE, Jahrling PB, Marasco WA. 3B11-N, a monoclonal antibody against MERS-CoV, reduces lung pathology in rhesus monkeys following intratracheal inoculation of MERS-CoV Jordan-n3/2012. Virology. 2016 03; 490:49-58.
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Chang DK, Peterson E, Sun J, Goudie C, Drapkin RI, Liu JF, Matulonis U, Zhu Q, Marasco WA. Anti-CCR4 monoclonal antibody enhances antitumor immunity by modulating tumor-infiltrating Tregs in an ovarian cancer xenograft humanized mouse model. Oncoimmunology. 2016 Mar; 5(3):e1090075.
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Menachery VD, Yount BL, Debbink K, Agnihothram S, Gralinski LE, Plante JA, Graham RL, Scobey T, Ge XY, Donaldson EF, Randell SH, Lanzavecchia A, Marasco WA, Shi ZL, Baric RS. A SARS-like cluster of circulating bat coronaviruses shows potential for human emergence. Nat Med. 2015 Dec; 21(12):1508-13.
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Freund NT, Roitburd-Berman A, Sui J, Marasco WA, Gershoni JM. Reconstitution of the receptor-binding motif of the SARS coronavirus. Protein Eng Des Sel. 2015 Dec; 28(12):567-75.
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Tang XC, Marasco WA. Human neutralizing antibodies against MERS coronavirus: implications for future immunotherapy. Immunotherapy. 2015; 7(6):591-4.
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Chang DK, Moniz RJ, Xu Z, Sun J, Signoretti S, Zhu Q, Marasco WA. Human anti-CAIX antibodies mediate immune cell inhibition of renal cell carcinoma in vitro and in a humanized mouse model in vivo. Mol Cancer. 2015 Jun 11; 14:119.
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Huang X, Dong W, Milewska A, Golda A, Qi Y, Zhu QK, Marasco WA, Baric RS, Sims AC, Pyrc K, Li W, Sui J. Human Coronavirus HKU1 Spike Protein Uses O-Acetylated Sialic Acid as an Attachment Receptor Determinant and Employs Hemagglutinin-Esterase Protein as a Receptor-Destroying Enzyme. J Virol. 2015 Jul; 89(14):7202-13.
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Goettel JA, Biswas S, Lexmond WS, Yeste A, Passerini L, Patel B, Yang S, Sun J, Ouahed J, Shouval DS, McCann KJ, Horwitz BH, Mathis D, Milford EL, Notarangelo LD, Roncarolo MG, Fiebiger E, Marasco WA, Bacchetta R, Quintana FJ, Pai SY, Klein C, Muise AM, Snapper SB. Fatal autoimmunity in mice reconstituted with human hematopoietic stem cells encoding defective FOXP3. Blood. 2015 Jun 18; 125(25):3886-95.
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Sheehan J, Marasco WA. Phage and Yeast Display. Microbiol Spectr. 2015 Feb; 3(1):AID-0028-2014.
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Lo AS, Xu C, Murakami A, Marasco WA. Regression of established renal cell carcinoma in nude mice using lentivirus-transduced human T cells expressing a human anti-CAIX chimeric antigen receptor. Mol Ther Oncolytics. 2014; 1:14003.
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Kang W, Marasco WA, Tong HI, Byron MM, Wu C, Shi Y, Sun S, Sun Y, Lu Y. Anti-tat Hutat2:Fc mediated protection against tat-induced neurotoxicity and HIV-1 replication in human monocyte-derived macrophages. J Neuroinflammation. 2014 Nov 22; 11:195.
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Sui J, Deming M, Rockx B, Liddington RC, Zhu QK, Baric RS, Marasco WA. Effects of human anti-spike protein receptor binding domain antibodies on severe acute respiratory syndrome coronavirus neutralization escape and fitness. J Virol. 2014 Dec; 88(23):13769-80.
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Abdel-Motal UM, Harbison C, Han T, Pudney J, Anderson DJ, Zhu Q, Westmoreland S, Marasco WA. Prolonged expression of an anti-HIV-1 gp120 minibody to the female rhesus macaque lower genital tract by AAV gene transfer. Gene Ther. 2014 Sep; 21(9):802-10.
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Avnir Y, Tallarico AS, Zhu Q, Bennett AS, Connelly G, Sheehan J, Sui J, Fahmy A, Huang CY, Cadwell G, Bankston LA, McGuire AT, Stamatatos L, Wagner G, Liddington RC, Marasco WA. Molecular signatures of hemagglutinin stem-directed heterosubtypic human neutralizing antibodies against influenza A viruses. PLoS Pathog. 2014 May; 10(5):e1004103.
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Tang XC, Agnihothram SS, Jiao Y, Stanhope J, Graham RL, Peterson EC, Avnir Y, Tallarico AS, Sheehan J, Zhu Q, Baric RS, Marasco WA. Identification of human neutralizing antibodies against MERS-CoV and their role in virus adaptive evolution. Proc Natl Acad Sci U S A. 2014 May 13; 111(19):E2018-26.
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Almagro JC, Gilliland GL, Breden F, Scott JK, Sok D, Pauthner M, Reichert JM, Helguera G, Andrabi R, Mabry R, Bléry M, Voss JE, Laurén J, Abuqayyas L, Barghorn S, Ben-Jacob E, Crowe JE, Huston JS, Johnston SA, Krauland E, Lund-Johansen F, Marasco WA, Parren PW, Xu KY. Antibody engineering and therapeutics, The Annual Meeting of the Antibody Society: December 8-12, 2013, Huntington Beach, CA. MAbs. 2014 May-Jun; 6(3):577-618.
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Hall SR, Jiang Y, Leary E, Yavanian G, Eminli S, O'Neill DW, Marasco WA. Identification and isolation of small CD44-negative mesenchymal stem/progenitor cells from human bone marrow using elutriation and polychromatic flow cytometry. Stem Cells Transl Med. 2013 Aug; 2(8):567-78.
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Zhang J, Valianou M, Simmons H, Robinson MK, Lee HO, Mullins SR, Marasco WA, Adams GP, Weiner LM, Cheng JD. Identification of inhibitory scFv antibodies targeting fibroblast activation protein utilizing phage display functional screens. FASEB J. 2013 Feb; 27(2):581-9.
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Han T, Abdel-Motal UM, Chang DK, Sui J, Muvaffak A, Campbell J, Zhu Q, Kupper TS, Marasco WA. Human anti-CCR4 minibody gene transfer for the treatment of cutaneous T-cell lymphoma. PLoS One. 2012; 7(9):e44455.
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Braun SE, Taube R, Zhu Q, Wong FE, Murakami A, Kamau E, Dwyer M, Qiu G, Daigle J, Carville A, Johnson RP, Marasco WA. In vivo selection of CD4(+) T cells transduced with a gamma-retroviral vector expressing a single-chain intrabody targeting HIV-1 tat. Hum Gene Ther. 2012 Sep; 23(9):917-31.
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Vyas JM, Marasco WA. Fatal fulminant hepatic failure from adenovirus in allogeneic bone marrow transplant patients. Case Rep Infect Dis. 2012; 2012:463569.
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Chang DK, Sui J, Geng S, Muvaffak A, Bai M, Fuhlbrigge RC, Lo A, Yammanuru A, Hubbard L, Sheehan J, Campbell JJ, Zhu Q, Kupper TS, Marasco WA. Humanization of an anti-CCR4 antibody that kills cutaneous T-cell lymphoma cells and abrogates suppression by T-regulatory cells. Mol Cancer Ther. 2012 Nov; 11(11):2451-61.
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Hammond SP, Gagne LS, Stock SR, Marty FM, Gelman RS, Marasco WA, Poritz MA, Baden LR. Respiratory virus detection in immunocompromised patients with FilmArray respiratory panel compared to conventional methods. J Clin Microbiol. 2012 Oct; 50(10):3216-21.
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Chang H, Biswas S, Tallarico AS, Sarkis PT, Geng S, Panditrao MM, Zhu Q, Marasco WA. Human B-cell ontogeny in humanized NOD/SCID ?c(null) mice generates a diverse yet auto/poly- and HIV-1-reactive antibody repertoire. Genes Immun. 2012 Jul; 13(5):399-410.
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Biswas S, Chang H, Sarkis PT, Fikrig E, Zhu Q, Marasco WA. Humoral immune responses in humanized BLT mice immunized with West Nile virus and HIV-1 envelope proteins are largely mediated via human CD5+ B cells. Immunology. 2011 Dec; 134(4):419-33.
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Abdel-Motal UM, Sarkis PT, Han T, Pudney J, Anderson DJ, Zhu Q, Marasco WA. Anti-gp120 minibody gene transfer to female genital epithelial cells protects against HIV-1 virus challenge in vitro. PLoS One. 2011; 6(10):e26473.
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Han T, Sui J, Bennett AS, Liddington RC, Donis RO, Zhu Q, Marasco WA. Fine epitope mapping of monoclonal antibodies against hemagglutinin of a highly pathogenic H5N1 influenza virus using yeast surface display. Biochem Biophys Res Commun. 2011 Jun 03; 409(2):253-9.
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Sui J, Sheehan J, Hwang WC, Bankston LA, Burchett SK, Huang CY, Liddington RC, Beigel JH, Marasco WA. Wide prevalence of heterosubtypic broadly neutralizing human anti-influenza A antibodies. Clin Infect Dis. 2011 Apr 15; 52(8):1003-9.
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Han T, Marasco WA. Structural basis of influenza virus neutralization. Ann N Y Acad Sci. 2011 Jan; 1217:178-90.
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Wrammert J, Koutsonanos D, Li GM, Edupuganti S, Sui J, Morrissey M, McCausland M, Skountzou I, Hornig M, Lipkin WI, Mehta A, Razavi B, Del Rio C, Zheng NY, Lee JH, Huang M, Ali Z, Kaur K, Andrews S, Amara RR, Wang Y, Das SR, O'Donnell CD, Yewdell JW, Subbarao K, Marasco WA, Mulligan MJ, Compans R, Ahmed R, Wilson PC. Broadly cross-reactive antibodies dominate the human B cell response against 2009 pandemic H1N1 influenza virus infection. J Exp Med. 2011 Jan 17; 208(1):181-93.
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Hashem AM, Van Domselaar G, Li C, Wang J, She YM, Cyr TD, Sui J, He R, Marasco WA, Li X. Universal antibodies against the highly conserved influenza fusion peptide cross-neutralize several subtypes of influenza A virus. Biochem Biophys Res Commun. 2010 Dec 10; 403(2):247-51.
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Herschhorn A, Marasco WA, Hizi A. Antibodies and lentiviruses that specifically recognize a T cell epitope derived from HIV-1 Nef protein and presented by HLA-C. J Immunol. 2010 Dec 15; 185(12):7623-32.
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Kuan CT, Srivastava N, McLendon RE, Marasco WA, Zalutsky MR, Bigner DD. Recombinant single-chain variable fragment antibodies against extracellular epitopes of human multidrug resistance protein MRP3 for targeting malignant gliomas. Int J Cancer. 2010 Aug 01; 127(3):598-611.
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Xu C, Lo A, Yammanuru A, Tallarico AS, Brady K, Murakami A, Barteneva N, Zhu Q, Marasco WA. Unique biological properties of catalytic domain directed human anti-CAIX antibodies discovered through phage-display technology. PLoS One. 2010 Mar 10; 5(3):e9625.
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Centeno CJ, Schultz JR, Cheever M, Robinson B, Freeman M, Marasco W. Safety and complications reporting on the re-implantation of culture-expanded mesenchymal stem cells using autologous platelet lysate technique. Curr Stem Cell Res Ther. 2010 Mar; 5(1):81-93.
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Sultana H, Foellmer HG, Neelakanta G, Oliphant T, Engle M, Ledizet M, Krishnan MN, Bonafé N, Anthony KG, Marasco WA, Kaplan P, Montgomery RR, Diamond MS, Koski RA, Fikrig E. Fusion loop peptide of the West Nile virus envelope protein is essential for pathogenesis and is recognized by a therapeutic cross-reactive human monoclonal antibody. J Immunol. 2009 Jul 01; 183(1):650-60.
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Gennari F, Lopes L, Verhoeyen E, Marasco W, Collins MK. Single-chain antibodies that target lentiviral vectors to MHC class II on antigen-presenting cells. Hum Gene Ther. 2009 Jun; 20(6):554-62.
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Sui J, Hwang WC, Perez S, Wei G, Aird D, Chen LM, Santelli E, Stec B, Cadwell G, Ali M, Wan H, Murakami A, Yammanuru A, Han T, Cox NJ, Bankston LA, Donis RO, Liddington RC, Marasco WA. Structural and functional bases for broad-spectrum neutralization of avian and human influenza A viruses. Nat Struct Mol Biol. 2009 Mar; 16(3):265-73.
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Nakase K, Cheng J, Zhu Q, Marasco WA. Mechanisms of SHP-1 P2 promoter regulation in hematopoietic cells and its silencing in HTLV-1-transformed T cells. J Leukoc Biol. 2009 Jan; 85(1):165-74.
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Sui J, Aird DR, Tamin A, Murakami A, Yan M, Yammanuru A, Jing H, Kan B, Liu X, Zhu Q, Yuan QA, Adams GP, Bellini WJ, Xu J, Anderson LJ, Marasco WA. Broadening of neutralization activity to directly block a dominant antibody-driven SARS-coronavirus evolution pathway. PLoS Pathog. 2008 Nov; 4(11):e1000197.
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Taube R, Zhu Q, Xu C, Diaz-Griffero F, Sui J, Kamau E, Dwyer M, Aird D, Marasco WA. Lentivirus display: stable expression of human antibodies on the surface of human cells and virus particles. PLoS One. 2008 Sep 11; 3(9):e3181.
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Huang IC, Li W, Sui J, Marasco W, Choe H, Farzan M. Influenza A virus neuraminidase limits viral superinfection. J Virol. 2008 May; 82(10):4834-43.
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Rodgerson D, Rothenberg R and Marasco WA. . The Collection, Processing and Storage of Adult Stem Cells – A Bio-Insurance Policy for Personalized Medical Therapies. Life Extension Foundation magazine. 2008.
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Lo AS, Zhu Q, Marasco WA. Intracellular antibodies (intrabodies) and their therapeutic potential. Handb Exp Pharmacol. 2008; (181):343-73.
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Marasco WA, Sui J. The growth and potential of human antiviral monoclonal antibody therapeutics. Nat Biotechnol. 2007 Dec; 25(12):1421-34.
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Xu C, Sui J, Tao H, Zhu Q, Marasco WA. Human anti-CXCR4 antibodies undergo VH replacement, exhibit functional V-region sulfation, and define CXCR4 antigenic heterogeneity. J Immunol. 2007 Aug 15; 179(4):2408-18.
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Li W, Sui J, Huang IC, Kuhn JH, Radoshitzky SR, Marasco WA, Choe H, Farzan M. The S proteins of human coronavirus NL63 and severe acute respiratory syndrome coronavirus bind overlapping regions of ACE2. Virology. 2007 Oct 25; 367(2):367-74.
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Cheng J, Kydd AR, Nakase K, Noonan KM, Murakami A, Tao H, Dwyer M, Xu C, Zhu Q, Marasco WA. Negative regulation of the SH2-homology containing protein-tyrosine phosphatase-1 (SHP-1) P2 promoter by the HTLV-1 Tax oncoprotein. Blood. 2007 Sep 15; 110(6):2110-20.
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Lo A, Zhu QK and Marasco WA. . Intracellular antibodies (Intrabodies) as specific molecules for therapeutic applications. In Handbook of Experimental Pharmacology. Chernajovsky Y, ed. 2007.
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Hwang WC, Lin Y, Santelli E, Sui J, Jaroszewski L, Stec B, Farzan M, Marasco WA, Liddington RC. Structural basis of neutralization by a human anti-severe acute respiratory syndrome spike protein antibody, 80R. J Biol Chem. 2006 Nov 10; 281(45):34610-6.
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Kanai R, Kar K, Anthony K, Gould LH, Ledizet M, Fikrig E, Marasco WA, Koski RA, Modis Y. Crystal structure of west nile virus envelope glycoprotein reveals viral surface epitopes. J Virol. 2006 Nov; 80(22):11000-8.
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Bai J, Demirjian A, Sui J, Marasco W, Callery MP. Histone deacetylase inhibitor trichostatin A and proteasome inhibitor PS-341 synergistically induce apoptosis in pancreatic cancer cells. Biochem Biophys Res Commun. 2006 Oct 06; 348(4):1245-53.
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Yuan QA, Simmons HH, Robinson MK, Russeva M, Marasco WA, Adams GP. Development of engineered antibodies specific for the Müllerian inhibiting substance type II receptor: a promising candidate for targeted therapy of ovarian cancer. Mol Cancer Ther. 2006 Aug; 5(8):2096-105.
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Marasco WA*, Sui J. . Convergent Combination Immunotherapy for the Prevention and Treatment of SARS. The 5th International Congress on Recombinant Antibodies. Zurich, Switzerland. 2006.
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Si Z, Vandegraaff N, O'huigin C, Song B, Yuan W, Xu C, Perron M, Li X, Marasco WA, Engelman A, Dean M, Sodroski J. Evolution of a cytoplasmic tripartite motif (TRIM) protein in cows that restricts retroviral infection. Proc Natl Acad Sci U S A. 2006 May 09; 103(19):7454-9.
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Marasco WA*, Sui J. . Passive immunotherapy in an outbreak setting. A case of SARS. From Concept to Clinic. Cambridge Health Institute’s 7th Annual Meeting, Boston, MA. 2006.
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Tarnovitski N, Matthews LJ, Sui J, Gershoni JM, Marasco WA. Mapping a neutralizing epitope on the SARS coronavirus spike protein: computational prediction based on affinity-selected peptides. J Mol Biol. 2006 May 26; 359(1):190-201.
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Sui J and Marasco WA. . Immunopathogenesis and Immunotherapy of SARS. In Challenges of Severe Acute Respiratory Syndrome. Chan JCK and Wong VCW, eds. 2006.
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Sui J, Aird DR, Marasco WA*. . Human neutralizing antibodies for convergent immunotherapy against SARS. 16th Annual International Conference on Antibody Engineering, San Diego, CA. 2005.
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Gould LH, Sui J, Foellmer H, Oliphant T, Wang T, Ledizet M, Murakami A, Noonan K, Lambeth C, Kar K, Anderson JF, de Silva AM, Diamond MS, Koski RA, Marasco WA, Fikrig E. Protective and therapeutic capacity of human single-chain Fv-Fc fusion proteins against West Nile virus. J Virol. 2005 Dec; 79(23):14606-13.
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Marasco WA*. . A strategy to move potent neutralizing anti-SARS monoclonal antibodies into clinical trials in an outbreak setting. The Second China International Symposium on Antibody Engineering. Invited Speaker. Therapeutic Antibodies for Non-Malignant Diseases. Beijing, China,. 2005.
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Marasco WA*, Sui J. . A Strategy Toward Convergent Combination Immunotherapy for SARS. ICLAS-CALAS International Symposium. Animal Models of Infectious Diseases. Beijing, China. 2005.
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Lo AS-Y, Dwyer MR, Appleman LJ, Marasco WA*. . Development of Renal Cell Carcinoma (RCC) Vaccine against G250 by Co-Expression of G250 Extracellular Domain-Fc Fusion Protein and IL-2 Using 2A Self-Processing Peptide. CHI Conference. Cancer Immunotheerptics and Targeted Cancer Therapies, Boston, MA. 2005.
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Cheng J, Noonan K and Marasco WA*. . Suppresses Shp-1 (SH2-containing Tyrosine Phosphatase-1) Gene Expression and Facilitates Leukemogenesis Through Tax-Promoter Interaction. 12th International Conference on Human Retrovirology: HTLV-1 and Related Viruses. Montego Bay, Jamaica. 2005.
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Marasco, WA*, Cheng J, and Noonan K. . Role of HTLV-1 Tax in SH2-containing Tyrosine Phosphatase-1 (SHP-1) Promoter Silencing in Acute Infection and Adult T-Cell Leukemia. 12th International Conference on Human Retrovirology: HTLV-1 and Related Viruses. Montego Bay, Jamaica. 2005.
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Bal HP, Cheng J, Murakami A, Tallarico AS, Wang W, Zhou D, Vasicek TJ, Marasco WA. GITR overexpression on CD4+CD25+ HTLV-1 transformed cells: detection by massively parallel signature sequencing. Biochem Biophys Res Commun. 2005 Jul 01; 332(2):569-84.
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Qin C, Wang J, Wei Q, She M, Marasco WA, Jiang H, Tu X, Zhu H, Ren L, Gao H, Guo L, Huang L, Yang R, Cong Z, Guo L, Wang Y, Liu Y, Sun Y, Duan S, Qu J, Chen L, Tong W, Ruan L, Liu P, Zhang H, Zhang J, Zhang H, Liu D, Liu Q, Hong T, He W. An animal model of SARS produced by infection of Macaca mulatta with SARS coronavirus. J Pathol. 2005 Jul; 206(3):251-9.
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Song E, Zhu P, Lee SK, Chowdhury D, Kussman S, Dykxhoorn DM, Feng Y, Palliser D, Weiner DB, Shankar P, Marasco WA, Lieberman J. Antibody mediated in vivo delivery of small interfering RNAs via cell-surface receptors. Nat Biotechnol. 2005 Jun; 23(6):709-17.
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Sui J, Li W, Roberts A, Matthews LJ, Murakami A, Vogel L, Wong SK, Subbarao K, Farzan M, Marasco WA. Evaluation of human monoclonal antibody 80R for immunoprophylaxis of severe acute respiratory syndrome by an animal study, epitope mapping, and analysis of spike variants. J Virol. 2005 May; 79(10):5900-6.
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Marasco WA. Therapeutic antibody gene transfer. Nat Biotechnol. 2005 May; 23(5):551-2.
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Li W, Zhang C, Sui J, Kuhn JH, Moore MJ, Luo S, Wong SK, Huang IC, Xu K, Vasilieva N, Murakami A, He Y, Marasco WA, Guan Y, Choe H, Farzan M. Receptor and viral determinants of SARS-coronavirus adaptation to human ACE2. EMBO J. 2005 Apr 20; 24(8):1634-43.
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Bai J, Sui J, Demirjian A, Vollmer CM, Marasco W, Callery MP. Predominant Bcl-XL knockdown disables antiapoptotic mechanisms: tumor necrosis factor-related apoptosis-inducing ligand-based triple chemotherapy overcomes chemoresistance in pancreatic cancer cells in vitro. Cancer Res. 2005 Mar 15; 65(6):2344-52.
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Cheng J, Kydd A, Noonan K, Xu C, Murakami A, and Marasco WA*. . Role of SHP-1 deregulation in HTLV-1 leukemogenesis. 12th Conference on Retroviruses and Opportunistic Infections, Boston, MA. 2005.
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Marasco, WA*, Sui J, Xu C, Murakami A and Noonan K. . Potential Use of Human Monoclonal Antibodies Against CXCR4 for the Prevention and Treatment of Breast Cancer Metastasis. Keystone Symposium on Antibody Based Therapeutics for Cancer, Santa Fe, NM. 2005.
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Masiero S, Del Vecchio C, Gavioli R, Mattiuzzo G, Cusi MG, Micheli L, Gennari F, Siccardi A, Marasco WA, Palù G, Parolin C. T-cell engineering by a chimeric T-cell receptor with antibody-type specificity for the HIV-1 gp120. Gene Ther. 2005 Feb; 12(4):299-310.
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Marasco WA*. . A strategy for Global Collaborations to Develop Human Monoclonal Antibody (mAb) Drugs for the Treatment of Cancer. Oncology Research. 2005; 15:458.
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Gould LH, Sui J, Ledizet M, Foellmer H, Koski R, Marasco WA*, and Fikrig E. . Preventive and therapeutic immunization against lethal murine West Nile virus infection with a bivalent human single-chain antibody (scFv)-Fc fusion protein and rabbit polyclonal antibody against the envelope protein. American Association of Tropical Medicine and Hygiene Annual Meeting, Miami Beach, FL. 2004.
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Gennari F, Lopes L, Marasco WA* and Collins MK. . Targeting Dendritic Cells: Selection of Single Chain Antibodies from a Human Phage Display Library. The 12th Annual Congress of the European Society of Gene Therapy, Tampere, Finland. 2004.
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Busch A, Marasco WA, Doebis C, Volk HD, Seifert M. MHC class I manipulation on cell surfaces by gene transfer of anti-MHC class I intrabodies--a tool for decreased immunogenicity of allogeneic tissue and cell transplants. Methods. 2004 Oct; 34(2):240-9.
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Sui J, Xu C, Murakami A, Noonan K and Marasco WA*. . Isolation of human antibodies against CXCR4 by paramagnetic proteoliposomes for prevention and treatment of breast cancer metastases. The Susan G, Komen Cancer Foundation Pathways to a Promise. New York, NY. 2004.
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Bal HP, Cheng J, Murakami A, Wang W, Vasicek TJ, and Marasco WA*. . Transcriptional deregulation in adult T-cell leukemia: Genome-wide transcriptional profiling of acutely HTLV-1 transformed cells using Massively Parallel Signature Sequencing. 95th AACR Annual Meeting, Orlando, FL. 2004.
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Sui J, Li W, Murakami A, Tamin A, Matthews LJ, Wong SK, Moore MJ, Tallarico AS, Olurinde M, Choe H, Anderson LJ, Bellini WJ, Farzan M, Marasco WA. Potent neutralization of severe acute respiratory syndrome (SARS) coronavirus by a human mAb to S1 protein that blocks receptor association. Proc Natl Acad Sci U S A. 2004 Feb 24; 101(8):2536-41.
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Cheng JD, Valianou M, Simmons H, Jann P, Yuan QA, Russeva MG, Marasco WA*, Cukierman E, Adams GP, and Weiner LM. Abrogation of Fibroblast Activation Protein Enzymatic Activity Attenuates Tumor Growth. AACR Annual Meeting, Dana Point, CA. 2004.
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Cheng J, Kydd A, Noonan K, Xu C, Murakami A, and Marasco WA*. . Role of SHP-1 deregulation in HTLV-1 leukemogenesis. 11th Conference on Retroviruses and Opportunistic Infections, San Francisco, CA. 2004.
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Gennari F, Mehta S, Wang Y, St Clair Tallarico A, Palu G, Marasco WA. Direct phage to intrabody screening (DPIS): demonstration by isolation of cytosolic intrabodies against the TES1 site of Epstein Barr virus latent membrane protein 1 (LMP1) that block NF-kappaB transactivation. J Mol Biol. 2004 Jan 02; 335(1):193-207.
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Cheng J, Zhang D, Zhou C, Marasco WA. Down-regulation of SHP1 and up-regulation of negative regulators of JAK/STAT signaling in HTLV-1 transformed cell lines and freshly transformed human peripheral blood CD4+ T-cells. Leuk Res. 2004 Jan; 28(1):71-82.
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Sui J, Bai J, St Clair Tallarico A, Xu C, Marasco WA. Identification of CD4 and transferrin receptor antibodies by CXCR4 antibody-guided Pathfinder selection. Eur J Biochem. 2003 Nov; 270(22):4497-506.
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Vyas J and Marasco WA*. . Fatal Fulminant Hepatic Failure from Adenovirus in Allogeneic Bone Marrow Transplant Patients. 41st Annual Meeting of Infectious Diseases Society of America. 2003.
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Marty FM, Cosimi L, Marasco WA*, Rubin RH and Baden LR. . Breakthrough Zygomycosis in Allogeneic Hematopoietic Stem Cell Transplant Recipients Who Received Voriconazole as Prophylaxis or Empiric Therapy. 43rd ICAAC. 2003.
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Xu C, Kontos H, Tallarico A, Murakami A, Morse LJ, Cheng J and Marasco WA*. . Generation and Characterization of Engineered Human Anti-CCR5 Antibodies. 3rd Annual Harvard Medical School Centers for AIDS Research Symposium, Boston, MA. 2003.
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Cheng J, Zhang D, Zhou C and Marasco WA*. . Down-Regulation of SHP1 and Up-Regulation of Negative Regulators of JAK/STAT Signaling in HTLV-1 Transformed Cell Lines and Freshly Transformed Human Peripheral Blood CD4+ T-cells. 3rd Annual Harvard Medical School Centers for AIDS Research Symposium, Boston, MA. 2003.
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Zhang D, Murakami A, Johnson RP, Sui J, Cheng J, Bai J, Marasco WA. Optimization of ex vivo activation and expansion of macaque primary CD4-enriched peripheral blood mononuclear cells for use in anti-HIV immunotherapy and gene therapy strategies. J Acquir Immune Defic Syndr. 2003 Mar 01; 32(3):245-54.
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Marasco WA*, Murakami A, Gennari F. . Intrabodies as Tools to Perturb Cellular Function. Guest Speaker for the Antibody-Based Therapeutics for Cancer, 2003 Keystone Symposia. 2003.
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Zhu Q and Marasco WA. . Intracellular targeting of antibodies in mammalian cells. In New Comprehensive Biocehmistry : Gene Transfer and Expression in Mammalian Cells. Savvas C. Makrides , ed. 2003; 573 – 623.
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Sui J, Marasco WA. Evidence against Ebola virus sGP binding to human neutrophils by a specific receptor. Virology. 2002 Nov 10; 303(1):9-14.
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Bai J, Sui J, Zhu RY, Tallarico AS, Gennari F, Zhang D, Marasco WA. Inhibition of Tat-mediated transactivation and HIV-1 replication by human anti-hCyclinT1 intrabodies. J Biol Chem. 2003 Jan 17; 278(3):1433-42.
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Mhashilkar AM, Doebis C, Seifert M, Busch A, Zani C, Soo Hoo J, Nagy M, Ritter T, Volk HD, Marasco WA. Intrabody-mediated phenotypic knockout of major histocompatibility complex class I expression in human and monkey cell lines and in primary human keratinocytes. Gene Ther. 2002 Mar; 9(5):307-19.
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Li X, Josef J, Marasco WA. Hiv-1 Tat can substantially enhance the capacity of NIK to induce IkappaB degradation. Biochem Biophys Res Commun. 2001 Aug 24; 286(3):587-94.
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Li X, Stuckert P, Bosch I, Marks JD, Marasco WA. Single-chain antibody-mediated gene delivery into ErbB2-positive human breast cancer cells. Cancer Gene Ther. 2001 Aug; 8(8):555-65.
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Ogueta SB, Yao F, Marasco WA. Design and in vitro characterization of a single regulatory module for efficient control of gene expression in both plasmid DNA and a self-inactivating lentiviral vector. Mol Med. 2001 Aug; 7(8):569-79.
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Marasco WA. . Intrabodies as anti-viral agents. In Current Topics in Microbiology and Immunology. Dennis R. Burton,ed. 2001; 260:247-70.
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Marasco WA. Intrabodies as antiviral agents. Curr Top Microbiol Immunol. 2001; 260:247-70.
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Marasco, WA. Expression vectors and methods of use. 2000.
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Li X, Multon MC, Henin Y, Schweighoffer F, Venot C, Josef J, Zhou C, LaVecchio J, Stuckert P, Raab M, Mhashilkar A, Tocque B, Marasco WA. Grb3-3 is up-regulated in HIV-1-infected T-cells and can potentiate cell activation through NFATc. J Biol Chem. 2000 Oct 06; 275(40):30925-33.
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Li X, Multon MC, Henin Y, Schweighoffer F, Venot C, LaVecchio J, Josef J, Stuckert P, Mhashilkar A, Tocqué B, Marasco WA. Upregulation of the apoptosis-associated protein Grb3-3 in HIV-1-infected human CD4(+) lymphocytes. Biochem Biophys Res Commun. 2000 Sep 16; 276(1):362-70.
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Marasco, WA. Method of intracellular binding of target molecules. 2000.
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Mirzabekov T, Kontos H, Farzan M, Marasco W, Sodroski J. Paramagnetic proteoliposomes containing a pure, native, and oriented seven-transmembrane segment protein, CCR5. Nat Biotechnol. 2000 Jun; 18(6):649-54.
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Song SU, Marasco WA. Rapid establishment of myeloma cell lines expressing Fab(Tac)-protamine, a targetable protein vector, directed against high-affinity alpha-chain of human interleukin-2 receptor. Methods Mol Biol. 2000; 133:157-66.
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Song SU and Marasco WA. . Rapid establishment of myeloma cell lines expressing Fab (Tac)-Protamine, A targetable protein vector, directed against the high affinity alpha chain of the human interleukin-2 receptor. In Methods in Molecular Biology. Eric Kmiec, ed. 2000; 133:156-66.
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Marasco, WA. Intracellular targeting of endogenous proteins. 1999.
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Marasco WA, LaVecchio J, Winkler A. Human anti-HIV-1 tat sFv intrabodies for gene therapy of advanced HIV-1-infection and AIDS. J Immunol Methods. 1999 Dec 10; 231(1-2):223-38.
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Zhu Q, Zeng C, Huhalov A, Yao J, Turi TG, Danley D, Hynes T, Cong Y, DiMattia D, Kennedy S, Daumy G, Schaeffer E, Marasco WA, Huston JS. Extended half-life and elevated steady-state level of a single-chain Fv intrabody are critical for specific intracellular retargeting of its antigen, caspase-7. J Immunol Methods. 1999 Dec 10; 231(1-2):207-22.
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Poznansky MC, La Vecchio J, Silva-Arietta S, Porter-Brooks J, Brody K, Olszak IT, Adams GB, Ramstedt U, Marasco WA, Scadden DT. Inhibition of human immunodeficiency virus replication and growth advantage of CD4+ T cells and monocytes derived from CD34+ cells transduced with an intracellular antibody directed against human immunodeficiency virus type 1 Tat. Hum Gene Ther. 1999 Oct 10; 10(15):2505-14.
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Mhashilkar AM, LaVecchio J, Eberhardt B, Porter-Brooks J, Boisot S, Dove JH, Pumphrey C, Li X, Weissmahr RN, Ring DB, Ramstedt U, Marasco WA. Inhibition of human immunodeficiency virus type 1 replication in vitro in acutely and persistently infected human CD4+ mononuclear cells expressing murine and humanized anti-human immunodeficiency virus type 1 Tat single-chain variable fragment intrabodies. Hum Gene Ther. 1999 Jun 10; 10(9):1453-67.
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Marasco, WA. Reactive neutralizing human anti-gp120 recombinant antibody, DNA coding the same and use thereof. 1998.
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Marasco, WA. Method of intracellular binding of target molecules. 1998.
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Marasco, WA. Method of intracellular binding of target molecules. 1998.
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Marasco WA, Chen S, Richardson JH, Ramstedt U, Jones SD. Intracellular antibodies against HIV-1 envelope protein for AIDS gene therapy. Hum Gene Ther. 1998 Jul 20; 9(11):1627-42.
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Richardson JH, Hofmann W, Sodroski JG, Marasco WA. Intrabody-mediated knockout of the high-affinity IL-2 receptor in primary human T cells using a bicistronic lentivirus vector. Gene Ther. 1998 May; 5(5):635-44.
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Marasco WA. Antibodies for targeted gene therapy: extracellular gene targeting and intracellular expression. Adv Drug Deliv Rev. 1998 Apr 06; 31(1-2):153-170.
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Pumphrey CY, Marasco WA. The therapeutic potential of intracellular antibodies. BioDrugs. 1998 Mar; 9(3):179-85.
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Poznansky MC, Foxall R, Mhashilkar A, Coker R, Jones S, Ramstedt U, Marasco W. Inhibition of human immunodeficiency virus replication and growth advantage of CD4+ T cells from HIV-infected individuals that express intracellular antibodies against HIV-1 gp120 or Tat. Hum Gene Ther. 1998 Mar 01; 9(4):487-96.
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Vallat AV, De Girolami U, He J, Mhashilkar A, Marasco W, Shi B, Gray F, Bell J, Keohane C, Smith TW, Gabuzda D. Localization of HIV-1 co-receptors CCR5 and CXCR4 in the brain of children with AIDS. Am J Pathol. 1998 Jan; 152(1):167-78.
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Jones SD and Marasco WA. . Antibodies for targeted gene therapy: extracellular gene targeting and intracellular expression. In Advanced Drug Reviews. 1998; 31:153-170.
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Richardson JH, Waldmann TA, Sodroski JG, Marasco WA. Inducible knockout of the interleukin-2 receptor alpha chain: expression of the high-affinity IL-2 receptor is not required for the in vitro growth of HTLV-I-transformed cell lines. Virology. 1997 Oct 27; 237(2):209-16.
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Mhashilkar AM, Biswas DK, LaVecchio J, Pardee AB, Marasco WA. Inhibition of human immunodeficiency virus type 1 replication in vitro by a novel combination of anti-Tat single-chain intrabodies and NF-kappa B antagonists. J Virol. 1997 Sep; 71(9):6486-94.
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Levin R, Mhashilkar AM, Dorfman T, Bukovsky A, Zani C, Bagley J, Hinkula J, Niedrig M, Albert J, Wahren B, Göttlinger HG, Marasco WA. Inhibition of early and late events of the HIV-1 replication cycle by cytoplasmic Fab intrabodies against the matrix protein, p17. Mol Med. 1997 Feb; 3(2):96-110.
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Marasco WA. . Designing Intrabodies: Structural features and the use of intracellular trafficking signals. In Intrabodies: Basic Research and Clinical Gene Therapy Applications. Molecular Biology Intelligence Unit. Marasco WA, ed. 1997.
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Pumphrey CY and Marasco WA. . The therapeutic potential of intracellular antibodies. In BioDrugs. Beedle AS, ed. 1997.
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Mhashilkar AM and Marasco WA. . Intrabodies targeted against regulatory epitopes of HIV-1: Tat and Rev as two significant targets for gene therapy. In Intrabodies: Basic Research and Clinical Gene Therapy Applications. Molecular Biology Intelligence Unit. Marasco WA, ed. 1997.
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Rondon IJ, Marasco WA. Intracellular antibodies (intrabodies) for gene therapy of infectious diseases. Annu Rev Microbiol. 1997; 51:257-83.
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Rondon IJ and Marasco WA. . Gene therapy for HIV-1 using intracellular antibodies against HIV-1 Gag proteins. In Intrabodies: Basic Research and Clinical Gene Therapy Applications. Molecular Biology Intelligence Unit. Marasco WA, ed. 1997.
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Richardson JH and Marasco WA. . Studies on the phenotypic ¡§knockout¡¨ of the human interleukin-2 receptor ƒÑ chain. In Intrabodies: Basic Research and Clinical Gene Therapy Applications. Molecular Biology Intelligence Unit. Marasco WA, ed. 1997.
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Marasco WA. . Intrabodies: From antibody genes to intracellular immunization. In The Antibodies, Zanetti M and Capra JD, eds. 1997; 4.
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Jones SD and Marasco WA. . Intracellular antibodies (intrabodies): Potential applications in transgenic animal research and engineered resistance to pathogens. In Transgenic Animals - Generation and Use. Houdebine L-M, ed. . 1997; 74:501-6.
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Marasco WA. Intrabodies: turning the humoral immune system outside in for intracellular immunization. Gene Ther. 1997 Jan; 4(1):11-5.
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Chen JD, Yang Q, Yang AG, Marasco WA, Chen SY. Intra- and extracellular immunization against HIV-1 infection with lymphocytes transduced with an AAV vector expressing a human anti-gp120 antibody. Hum Gene Ther. 1996 Aug 20; 7(13):1515-25.
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Chen SY, Marasco WA. Novel genetic immunotoxins and intracellular antibodies for cancer therapy. Semin Oncol. 1996 Feb; 23(1):148-53.
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Marasco WA. . Gene Therapy and Research Applications of Intrabodies for Human Infectious Diseases. In The Use of Ectopic Antibody Expression for Intracellular and Extracellular Immunization. Biocca S. And Cattaneo A., eds. 1996.
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Mhashilkar AM, Agarwal S, Levin R, and Marasco WA. . Looking ahead: Combination of intracellular single-chain antibodies and other genetic-based anti-HIV-1 approaches hold promise as an effective strategy for AIDS gene therapy. In Drugs, News and Perspectives. 1996; 9:220-7.
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Mhashilkar AM and Marasco WA. . Intrabody-based gene therapy approaches against HIV-1. In -Advancing a New Agenda for AIDS Research, Poznansky M.C., Coker R.J. and Gilles P., eds. 1996.
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Richardson JH, Marasco WA. Intracellular antibodies: development and therapeutic potential. Trends Biotechnol. 1995 Aug; 13(8):306-10.
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Marasco WA. Intracellular antibodies (intrabodies) as research reagents and therapeutic molecules for gene therapy. Immunotechnology. 1995 May; 1(1):1-19.
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Richardson JH, Sodroski JG, Waldmann TA, Marasco WA. Phenotypic knockout of the high-affinity human interleukin 2 receptor by intracellular single-chain antibodies against the alpha subunit of the receptor. Proc Natl Acad Sci U S A. 1995 Apr 11; 92(8):3137-41.
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Mhashilkar AM, Bagley J, Chen SY, Szilvay AM, Helland DG, Marasco WA. Inhibition of HIV-1 Tat-mediated LTR transactivation and HIV-1 infection by anti-Tat single chain intrabodies. EMBO J. 1995 Apr 03; 14(7):1542-51.
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Chen SY, Zani C, Khouri Y, Marasco WA. Design of a genetic immunotoxin to eliminate toxin immunogenicity. Gene Ther. 1995 Mar; 2(2):116-23.
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Khouri YF, McIntosh K, Cavacini L, Posner M, Pagano M, Tuomala R, Marasco WA. Vertical Transmission of HIV-1. Correlation with maternal viral load and plasma levels of CD4 binding site anti-gp120 antibodies. J Clin Invest. 1995 Feb; 95(2):732-7.
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Becker EL and Marasco WA.* . Chemotaxis and activation of neutrophils. In Symposium on Chemical Mediators of Inflammation. Japan, Cohen S, Hayashi H, Saito K, and Takada A. eds. 1995; 220-4.
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Chen S-Y, Mhashilkar AM, and Marasco WA. . Intracellular antibodies for HIV-1 gene therapy. In Expert Opinion on Investigational Drugs. 1995; 4:823-31.
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Smith C, Marasco WA, and Böhnlein B. . Gene therapy. In HIV: A Practical Approach: Volume 2, Biochemistry, Molecular Biology, Drug Discovery. Karn J, ed. 1995.
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Bagley J, Dillon PJ, Rosen C, Robinson J, Sodroski J, Marasco WA. Structural characterization of broadly neutralizing human monoclonal antibodies against the CD4 binding site of HIV-1 gp120. Mol Immunol. 1994 Oct; 31(15):1149-60.
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Chen SY, Khouri Y, Bagley J, Marasco WA. Combined intra- and extracellular immunization against human immunodeficiency virus type 1 infection with a human anti-gp120 antibody. Proc Natl Acad Sci U S A. 1994 Jun 21; 91(13):5932-6.
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Chen SY, Bagley J, Marasco WA. Intracellular antibodies as a new class of therapeutic molecules for gene therapy. Hum Gene Ther. 1994 May; 5(5):595-601.
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Marasco WA, Szilvay AM, Kalland KH, Helland DG, Reyes HM, Walter RJ. Spatial association of HIV-1 tat protein and the nucleolar transport protein B23 in stably transfected Jurkat T-cells. Arch Virol. 1994; 139(1-2):133-54.
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Marasco WA, Haseltine WA, Chen SY. Design, intracellular expression, and activity of a human anti-human immunodeficiency virus type 1 gp120 single-chain antibody. Proc Natl Acad Sci U S A. 1993 Aug 15; 90(16):7889-93.
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Marasco WA, Bagley J, Zani C, Posner M, Cavacini L, Haseltine WA, Sodroski J. Characterization of the cDNA of a broadly reactive neutralizing human anti-gp120 monoclonal antibody. J Clin Invest. 1992 Oct; 90(4):1467-78.
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Garcia LW, Hemphill RB, Marasco WA, Ciano PS. Acquired immunodeficiency syndrome with disseminated toxoplasmosis presenting as an acute pulmonary and gastrointestinal illness. Arch Pathol Lab Med. 1991 May; 115(5):459-63.
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Bradley SF, Gordon JJ, Baumgartner DD, Marasco WA, Kauffman CA. Group C streptococcal bacteremia: analysis of 88 cases. Rev Infect Dis. 1991 Mar-Apr; 13(2):270-80.
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Sannomiya P, Craig RA, Clewell DB, Suzuki A, Fujino M, Till GO, Marasco WA. Characterization of a class of nonformylated Enterococcus faecalis-derived neutrophil chemotactic peptides: the sex pheromones. Proc Natl Acad Sci U S A. 1990 Jan; 87(1):66-70.
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Marasco WA, Lester S, Parsonnet J. Unusual presentation of cat scratch disease in a patient positive for antibody to the human immunodeficiency virus. Rev Infect Dis. 1989 Sep-Oct; 11(5):793-803.
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Janeczek AH, Marasco WA, van Alten PJ, Walter RJ. Autoradiographic analysis of formylpeptide chemoattractant binding, uptake and intracellular processing by neutrophils. J Cell Sci. 1989 Sep; 94 ( Pt 1):155-68.
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Feltner DE, Marasco WA. Regulation of formyl peptide receptor binding to rabbit neutrophil plasma membranes. Use of monovalent cations, guanine nucleotides, and bacterial toxins to discriminate among different states of the receptor. J Immunol. 1989 Jun 01; 142(11):3963-70.
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Atkinson YH, Lopez AF, Marasco WA, Lucas CM, Wong GG, Burns GF, Vadas MA. Recombinant human granulocyte-macrophage colony-stimulating factor (rH GM-CSF) regulates f Met-Leu-Phe receptors on human neutrophils. Immunology. 1988 Jul; 64(3):519-25.
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Atkinson YH, Marasco WA, Lopez AF, Vadas MA. Recombinant human tumor necrosis factor-alpha. Regulation of N-formylmethionylleucylphenylalanine receptor affinity and function on human neutrophils. J Clin Invest. 1988 Mar; 81(3):759-65.
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Marasco WA, Ward PA. Chemotactic factors of bacterial origin. Methods Enzymol. 1988; 162:198-214.
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Marasco WA and Ward PA. . Chemotactic factors of bacterial origin. Chapter 19. In Immunological Techniques. Part L. Chemotaxis and Inflammation. Methods in Enzymology. DiSabato G, Langone JJ, and VanVunakis H, eds. 1988; Vol. 162:198-214.
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Marasco WA, Gikas PW, Azziz-Baumgartner R, Hyzy R, Eldredge CJ, Stross J. Ibuprofen-associated renal dysfunction. Pathophysiologic mechanisms of acute renal failure, hyperkalemia, tubular necrosis, and proteinuria. Arch Intern Med. 1987 Dec; 147(12):2107-16.
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Walter RJ, Marasco WA. Direct visualization of formylpeptide receptor binding on rounded and polarized human neutrophils: cellular and receptor heterogeneity. J Leukoc Biol. 1987 May; 41(5):377-91.
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Walter RJ and Marasco WA. . Heterogeneity and topographical asymmetry of formyl peptide receptor binding on polymorphonuclear leukocytes. In The Biology of Phagocytes in Health and Disease. Mauri C, Rizzo SC, and Ricevuti G, eds. 1987; 9-21.
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Feltner DE, Smith RH, Marasco WA. Characterization of the plasma membrane bound GTPase from rabbit neutrophils. I. Evidence for an Ni-like protein coupled to the formyl peptide, C5a, and leukotriene B4 chemotaxis receptors. J Immunol. 1986 Sep 15; 137(6):1961-70.
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Duque RE, Fantone JC, Kramer C, Marasco WA, Phan SH. Inhibition of neutrophil activation by p-bromophenacyl bromide and its effects on phospholipase A2. Br J Pharmacol. 1986 Jun; 88(2):463-72.
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Marasco WA, Becker KM, Feltner DE, Brown CS, Ward PA, Nairn R. Covalent affinity labeling, detergent solubilization, and fluid-phase characterization of the rabbit neutrophil formyl peptide chemotaxis receptor. Biochemistry. 1985 Apr 23; 24(9):2227-36.
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Marasco WA, Ward PA, Feltner DE, Varani J. Chemotactic factor binding by metastatic tumour cells: evidence for a formyl-peptide receptor on a non-myelogenous cell. J Cell Sci. 1985 Feb; 73:121-34.
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Marasco WA, Feltner DE, Ward PA. Formyl peptide chemotaxis receptors on the rat neutrophil: experimental evidence for negative cooperativity. J Cell Biochem. 1985; 27(4):359-75.
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Marasco WA, Charles J, and Ward PA. . A search for "Internal Image" anti-idiotypic antibodies: A subpopulation of anti-f Met-Leu-Phe anti-idiotypic antibodies binds to the formyl peptide chemotaxis receptor of the neutrophil and to several genetically unrelated anti-f Met-Leu-Phe antibodies. In UCLA Symposium on Molecular and Cellular Biology. New Series, Reisfeld RA and Sell S. eds. 1985; 27:327-44.
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Nairn R, Smith RH, Brown CS, Marasco WA. What are the molecular characteristics of the neutrophil receptor for chemotactic formylated peptides? Surv Immunol Res. 1985; 4(3):213-9.
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Walter RJ, Marasco WA. Localization of chemotactic peptide receptors on rabbit neutrophils. Exp Cell Res. 1984 Oct; 154(2):613-8.
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Marasco WA, Phan SH, Krutzsch H, Showell HJ, Feltner DE, Nairn R, Becker EL, Ward PA. Purification and identification of formyl-methionyl-leucyl-phenylalanine as the major peptide neutrophil chemotactic factor produced by Escherichia coli. J Biol Chem. 1984 May 10; 259(9):5430-9.
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Fantone JC, Marasco WA, Elgas LJ, Ward PA. Stimulus specificity of prostaglandin inhibition of rabbit polymorphonuclear leukocyte lysosomal enzyme release and superoxide anion production. Am J Pathol. 1984 Apr; 115(1):9-16.
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Kunkel SL, Manderino GL, Marasco W, Kaercher K, Hirata AA, Ward PA. A specific enzyme-linked immunosorbent assay (ELISA) for the determination of human C5a antigen. J Immunol Methods. 1983 Sep 16; 62(3):305-14.
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Marasco WA, Fantone JC, Freer RJ, Ward PA. Characterization of the rat neutrophil formyl peptide chemotaxis receptor. Am J Pathol. 1983 Jun; 111(3):273-81.
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Fantone JC, Marasco WA, Elgas LJ, Ward PA. Anti-inflammatory effects of prostaglandin E1: in vivo modulation of the formyl peptide chemotactic receptor on the rat neutrophil. J Immunol. 1983 Apr; 130(4):1495-7.
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Marasco WA, Fantone JC, Ward PA. Spasmogenic activity of chemotactic N-formylated oligopeptides: identity of structure--function relationships for chemotactic and spasmogenic activities. Proc Natl Acad Sci U S A. 1982 Dec; 79(23):7470-3.
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Marasco WA, Showell HJ, Freer RJ, Becker EL. Anti-f Met-Leu-Phe: similarities in fine specificity with the formyl peptide chemotaxis receptor of the neutrophil. J Immunol. 1982 Feb; 128(2):956-62.
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Marasco WA, Becker EL. Anti-idiotype as antibody against the formyl peptide chemotaxis receptor of the neutrophil. J Immunol. 1982 Feb; 128(2):963-8.
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Marasco WA, Showell HJ, Becker EL. Substance P binds to the formylpeptide chemotaxis receptor on the rabbit neutrophil. Biochem Biophys Res Commun. 1981 Apr 30; 99(4):1065-72.
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Marasco WA, Becker EL, Oliver JM. The ionic basis of chemotaxis. Separate cation requirements for neutrophil orientation and locomotion in a gradient of chemotactic peptide. Am J Pathol. 1980 Mar; 98(3):749-68.
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