A Phase II Trial of PD-L1 therapy combined with anti-VEGF therapy in Unresectable or Metastatic Melanoma

This research study is studying a combination of two drugs that change the immune system and
tumor as a possible treatment for metastatic or unresectable stage III or IV cutaneous
melanoma.

The names of the study drugs involved in this study are:

- Atezolizumab

- Bevacizumab

Inclusion Criteria:

- Participants must have histologically or cytologically confirmed metastatic or
unresectable stage III or IV cutaneous melanoma.

- PD-L1 negative staining in at least one biopsy sample.

- Age ≥ 18 years

- ECOG performance status ≤ 2 (Karnofsky ≥60%, see Appendix A)

- Participants may have received any number of prior therapies for treatment of their
cutaneous melanoma, excluding prior treatment with anti-PD-L1 therapeutic antibodies
or bevacizumab.

- Participants must have measurable disease per RECIST v1.1.

- Participants must have availability of a representative tumor specimen for exploratory
biomarker research.

- Participants must have normal organ and marrow function as defined below:

- lymphocyte count ≥ 500/mcL

- absolute neutrophil count ≥ 1,500/mcL without granulocyte colony-stimulating
factor support

- platelets* ≥100,000/mcL

- hemoglobin* ≥9 g/dL

- serum bilirubin ≤1.5 x ULN with the following exception: participants with known
Gilbert's Disease: serum bilirubin level ≤ 3x ULN

- AST(SGOT)/ALT(SGPT) ≤2.5 × institutional ULN, with the following exceptions:
Participants with documented liver metastases: AST and ALT ≤ 5 x ULN

- alkaline phosphatase (ALP) ≤2.5 × institutional ULN, with the following
exceptions:

- participants with documented liver or bone metastases: ALP ≤ 5 x ULN

- serum creatinine ≤ 1.5 x ULN OR creatinine clearance ≥ 60 mL/min/1.73 m2 for
participants with creatinine levels above institutional normal.

- serum albumin ≥ 2.5 g/dL

- For participants not receiving therapeutic anticoagulation: INR or aPTT ≤1.5 ULN

- Urine dipstick for proteinuria < 2+ (within 7 days prior to Cycle 1 Day 1).
Participants discovered to have ≥ 2 + proteinuria on dipstick urinalysis at baseline
should undergo a 24-hour urine collection and must demonstrate < 1 g of protein in 24
hours. Note: Participants cannot be transfused to meet this criterion

- For women of childbearing potential: agreement to remain abstinent (refrain from
heterosexual intercourse) or use contraceptive methods with a failure rate of < 1% per
year during the treatment period and for 5 months after the last dose of atezolizumab,
and 6 months after the last dose of bevacizumab.

- Examples of contraceptive methods with a failure rate of < 1 % per year include
bilateral tubal ligation, male sterilization, hormonal contraceptives that inhibit
ovulation, hormone-releasing intrauterine devices, and copper intrauterine devices.

- The reliability of sexual abstinence should be evaluated in relation to the duration
of the clinical trial and the preferred and usual lifestyle of the patient. Periodic
abstinence (e.g., calendar, ovulation, symptothermal, or postovulation methods) and
withdrawal are not acceptable methods of contraception.

- For men: agreement to remain abstinent (refrain from heterosexual intercourse) or use
a condom, and agreement to refrain from donating sperm, as defined below: With female
partners of childbearing potential or pregnant female partners, men must remain
abstinent or use a condom during the treatment period and for 5 months after the last
dose of atezolizumab and 6 months after the last dose of bevacizumab, to avoid
exposing the embryo. Men must refrain from donating sperm during this same period. The
reliability of sexual abstinence should be evaluated in relation to the duration of
the clinical trial and the preferred and usual lifestyle of the patient. Periodic
abstinence (e.g., calendar, ovulation, symptothermal, or postovulation methods) and
withdrawal are not acceptable methods of contraception.

- Ability to understand and the willingness to sign a written informed consent document.

Exclusion Criteria:

- Participants with symptomatic, untreated, or actively progressing CNS metastases will
be excluded. If a participant has a known history of treated CNS lesions, they are
eligible provided that all of the following criteria are met:

- Measurable disease, per RECIST v1.1, must be present outside the CNS.

- The participant has no history of intracranial hemorrhage or spinal cord hemorrhage.

- Metastases are limited to the cerebellum or the supratentorial region (i.e., no
metastases to the midbrain, pons, medulla, or spinal cord).

- There is no evidence of interim progression between completion of CNS-directed therapy
and the screening brain scan

- The participant has not received stereotactic radiotherapy within 7 days prior to
initiation of study treatment or whole-brain radiotherapy within 14 days prior to
initiation of study treatment.

- The participant has no ongoing requirement for corticosteroids as therapy for CNS
disease. Anticonvulsant therapy at a stable dose is permitted. Asymptomatic patients
with CNS metastases newly detected at screening are eligible for the study after
receiving radiotherapy or surgery, with no need to repeat the screening brain scan.

- History of leptomeningeal disease.

- Uncontrolled tumor-related pain.

- NOTE: Participants requiring pain medication must be on a stable regimen at study
entry.

- Symptomatic lesions amendable to palliative radiotherapy (e.g., bone metastases
or metastases causing nerve impingement) should be treated prior to enrollment.
Participants should be recovered from the effects of radiation. There is no
required minimum recovery period.

- Asymptomatic metastatic lesions that would likely cause functional deficits or
intractable pain with further growth (e.g., epidural metastasis that is not
currently associated with spinal cord compression) should be considered for
loco-regional therapy if appropriate prior to enrollment.

- History of allergic reactions attributed to compounds of similar chemical or biologic
composition to atezolizumab and/or bevacizumab.

- Uncontrolled or symptomatic hypercalcemia (> 1.5 mmol/L ionized calcium or calcium >
12 mg/dL or corrected serum calcium > ULN)

- Active or history of autoimmune disease or immune deficiency, including, but not
limited to, myasthenia gravis, myositis, autoimmune hepatitis, systemic lupus
erythematosus, rheumatoid arthritis, inflammatory bowel disease, antiphospholipid
antibody syndrome,Wegener granulomatosis, Sjögren's syndrome, Guillain-Barré syndrome,
or multiple sclerosis with the following exceptions:

- Patients with a history of autoimmune-related hypothyroidism who are on
thyroid-replacement hormone are eligible for the study.

- Patients with controlled Type 1 diabetes mellitus who are on an insulin regimen
are eligible for the study.

- Patients with eczema, psoriasis, lichen simplex chronicus, or vitiligo with
dermatologic manifestations only (e.g., patients with psoriatic arthritis are
excluded) are eligible for the study provided all of following conditions are
met: Rash must cover <10% of body surface area, Disease is well controlled at
baseline and requires only low-potency topical corticosteroids, No occurrence of
acute exacerbations of the underlying condition requiring psoralen plus
ultraviolet. A radiation, methotrexate,retinoids, biologic agents, oral
calcineurin inhibitors, or high-potency or oral corticosteroids within the
previous 12 months.

- History of idiopathic pulmonary fibrosis, organizing pneumonia (e.g., bronchiolitis
obliterans), drug-induced pneumonitis, or idiopathic pneumonitis, or evidence of
active pneumonitis on screening chest computed tomography (CT) scan. History of
radiation pneumonitis in the radiation field (fibrosis) is permitted.

- Participants who test positive for HIV at time of screening are ineligible because of
the increased risk of lethal infections when treated with marrow-suppressive therapy.

- Active hepatitis B virus (HBV) infection (chronic or acute), defined as having a
positive hepatitis B surface antigen (HBsAg) test at screening. Participants with a
negative HBsAg test and a positive total hepatitis B core antibody (HBcAb) test at
screening are eligible for the study.

- Active hepatitis C virus (HCV) infection, defined as a positive HCV antibody test
followed by a positive HCV RNA test at screening. The HCV RNA test will be performed
only for participants who have a positive HCV antibody test.

- Participants with active tuberculosis will be excluded.

- Significant cardiovascular disease, such as a New York Heart Association Class II or
greater cardiac disease, myocardial infarction, or cerebrovascular accident within 3
months prior to initiation of study treatment, unstable arrhythmia, or unstable
angina.

- Major surgical procedure other than for diagnosis within 4 weeks prior to initiation
of study treatment, or anticipation of need for a major surgical procedure during the
course of the study.

- History of other malignancy within 2 years prior to screening, with the exception of
those with a negligible risk of metastasis or death (e.g., 5-year OS of > 90%), such
as adequately treated carcinoma in situ of the cervix, non-melanoma skin carcinoma,
localized prostate cancer, ductal carcinoma in situ, or Stage I uterine cancer.

- Severe infection within 4 weeks prior to initiation of study treatment, including, but
not limited to, hospitalization for complications of infection, bacteremia, or severe
pneumonia.

- Treatment with therapeutic oral or IV antibiotics within 2 weeks prior to initiation
of study treatment. Patients receiving prophylactic antibiotics (e.g., to prevent a
urinary tract infection or chronic obstructive pulmonary disease exacerbation) are
eligible for the study.

- Any other disease, metabolic dysfunction, physical examination finding, or clinical
laboratory finding that contraindicates the use of an investigational drug, may affect
the interpretation of the results, or may render the patient at high risk from
treatment complications.

- Prior allogeneic stem cell or solid organ transplantation.

- Treatment with a live, attenuated vaccine within 4 weeks prior to initiation of study
treatment, or anticipation of need for such a vaccine during the course of the study
or within 5 months after the last dose of atezolizumab.

- Treatment with investigational therapy within 28 days prior to initiation of study
treatment.

- Prior treatment with bevacizumab or prior PD-L1 targeted therapy.

- Treatment with systemic immunostimulatory agents (including, but not limited
to,interferon and interleukin 2 [IL-2]) within 4 weeks or five half-lives of the drug
(whichever is shorter) prior to initiation of study treatment.

- Treatment with systemic immunosuppressive medication (including, but not limited to,
corticosteroids, cyclophosphamide, azathioprine, methotrexate, thalidomide, and
anti-TNF-α agents) within 2 weeks prior to initiation of study treatment, or
anticipation of need for systemic immunosuppressive medication during the course of
the study, with the following exceptions:

- Patients who received acute, low-dose systemic immunosuppressant medication or a
one-time pulse dose of systemic immunosuppressant medication (e.g. 48 hours of
corticosteroids for a contrast allergy) are eligible for the study.

- Patients on physiologic replacement doses of steroids less than 10mg daily
prednisone equivalent are allowed. Higher replacement doses should be discussed
with the overall PI to determine if patient can be included on the trial.

- Patients who received mineralocorticoids (e.g., fludrocortisone), corticosteroids
for chronic obstructive pulmonary disease (COPD) or asthma, or low-dose
corticosteroids for orthostatic hypotension or adrenal insufficiency are eligible
for the study.

- History of severe allergic anaphylactic reactions to chimeric or humanized antibodies
or fusion proteins.

- Known hypersensitivity to Chinese hamster ovary cell products or to any component of
the atezolizumab formulation.

- Known allergy or hypersensitivity to any component of the bevacizumab formulation.

- Inadequately controlled arterial hypertension (defined as systolic blood pressure [BP]
> 150 mm Hg and/or diastolic BP >100 mmHg), based on an average of at least three BP
readings at two or more sessions. Anti-hypertensive therapy to achieve these
parameters is allowed.

- History of hypertensive crisis or hypertensive encephalopathy.

- Significant vascular disease (e.g., aortic aneurysm requiring surgical repair or
recent peripheral arterial thrombosis) within 6 months prior to Cycle 1 Day 1.

- History of hemoptysis (≥ 2.5 mL of bright red blood per episode) within 1 month prior
to Cycle 1 Day 1.

- Evidence of bleeding diathesis or significant coagulopathy (in the absence of
therapeutic anticoagulation).

- Current or recent (within 10 days of Cycle 1 Day 1) use of aspirin (> 325 mg/day) or
current or recent treatment with dipyramidole, ticlopidine, clopidogrel, and
cilostazol.

- Current or recent (within 10 days prior to Cycle 1 Day 1) use of full-dose oral or
parenteral anticoagulants or thrombolytic agents for therapeutic purpose (as opposed
to prophylactic). Prophylactic anticoagulation for the patency of venous access
devices is allowed provided the activity of the agent results in an INR < 1.5 x ULN
and aPTT is within normal limits (according to the institutional standards) within 14
days prior to Cycle 1 Day 1. Prophylactic use of low-molecular weight heparin (LMWH)
(i.e., enoxaparin 40 mg/day) is allowed. However, the use of direct oral anticoagulant
therapies such as dabigatran (Pradaxa®) and rivaroxaban (Xarelto®) is not recommended
due to bleeding risk.

- History of abdominal or tracheoesophageal fistula, GI perforation, or intra-abdominal
abscess within 6 months prior to Cycle 1 Day 1.

- Evidence of abdominal free air that is not explained by paracentesis or recent
surgical procedure.

- Serious, non-healing or dehiscing wound, active ulcer, or untreated bone fracture.

- Major surgical procedure within 4 weeks prior to Cycle 1 Day 1 or anticipation of need
for a major surgical procedure during the study.

- History of intra-abdominal inflammatory process within 6 months prior to Cycle 1 Day
1, including, but not limited to, peptic ulcer disease, diverticulitis, or colitis.

- Chronic daily treatment with a non-steroidal anti-inflammatory drug (NSAID).

- Pregnant or breastfeeding, or intending to become pregnant during the study or within
5 months after the last dose of atezolizumab and 6 months after the last dose of
bevacizumab. Women of childbearing potential must have a negative serum pregnancy test
result within 14 days prior to initiation of study treatment.