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CCGD Services

  • researchers at computers in a lab
  • Over the years, many of our collaborators have brought us archived FFPE samples for next-generation sequencing. As a result, we have acquired a great deal of experience with sub-optimal FFPE samples and have developed and validated techniques that consistently allow us to obtain high-quality data.

    In addition to our standard suite of services, CCGD often takes part in collaborations to develop and evaluate new NGS assays and techniques. As part of this process, we work with investigators in forging a research and development proposal and can assist in all aspects of research, including procurement of funding (e.g., grant submissions).

    Sample Types Accepted (Purified Nucleic Acid)

    • FFPE
    • Cell-free DNA (cfDNA)
    • Fresh tumor
    • Cell-line

    DNA Sequencing and Library Preparation (Illumina Platform)

    • Custom hybrid capture bait set design
    • Exome sequencing
    • Genome sequencing
    • Amplicon sequencing
    • Pre-designed hybrid capture bait panels (OncoPanel)

    Cell-Free DNA (cfDNA) Sequencing

    Liquid biopsies represent a readily accessible sample type upon which molecular profiling with NGS can be performed. CCGD has worked with several investigators to develop library construction methods tailored to the needs of cfDNA. We've recently replaced our library adapters with unique, dual-matched barcode adapters containing a unique molecular identifier (UMI) which allow us to:

    • Reduce barcode cross-talk
    • Form UMI read families to identify PCR errors
    • Discriminate between unique molecules of identical sequence and true PCR duplicates

    Current efforts are directed towards development of custom cfDNA hybrid capture gene panels.

    RNA Sequencing and Library Preparation (Stranded RNA-Seq)

    We are currently refining our library prep protocol to use FFPE samples and will be designing custom hybrid capture bait sets that will target both messenger RNA and fusion transcripts resulting from genomic rearrangements:

    • Expression analysis
    • Fusion transcript detection