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CPDM's Collection of Primary and Metastatic Brain Tumor PDX and Spheroid, Organoid Models

  • CPDM has a large collection of primary and metastatic brain tumor models (230+ PDCLs and 90+ PDXs).

    See our detailed model lists for more information about our brain tumor models. RNA-seq data, whole-exome seq data, and low-pass WGS data are available on cBioPortal.

    Learn more about ordering and licensing these models, or email us at models@dfci.harvard.edu to submit an inquiry.

    See CPDM's SOPs for working with these models for guidance.

    Primary Brain Tumor Models

    Our primary brain tumor models cover various gliomas and include several rare IDH1 R132H models, as well as rare models of oligodendroglioma, medulloblastoma, and pineoblastoma, atypical teratoid/rhabdoid tumor.


  • Organ Site Diagnosis # of Models
    Brain Glioblastoma IDH Wildtype 4 111
    Brain Glioblastoma NOS 4 33
    Brain Glioblastoma IDH Mutant 4 11
    Brain Anaplastic Oligodendroglioma IDH Mutant And 1P 19Q Codeleted 3 3
    Brain Medulloblastoma 4 6
    Brain Anaplastic Astrocytoma IDH Mutant 3 3
    Brain High Grade Glioma NOS 4
    Brain Anaplastic Oligoastrocytoma 3 4
    Brain Anaplastic Oligodendroglioma NOS 3 3
    Brain Meningioma Atypical 2 3
    Brain Pineoblastoma 4 2
    Brain Meningioma Anaplastic 3 2
    Brain Atypical Teratoid Rhabdoid Tumor 4 1
    Brain Diffuse Astrocytoma IDH Mutant 2 1

  • Many of the brain tumor models have been previously published in various studies including Cancer Cell 2011; Nature 2012; Br J Cancer 2014; Oncotarget 2015; Cell Rep. 2016; Cell 2016; Clin Cancer Res. 2016; Nat Biotechnol. 2016; Cell Rep. 2017; PNAS 2017; Cancer Res. 2018; Genome Biol. 2018; Nature Commun. 2019; Nature 2020; Ferrer-Luna et al., 2019 (in revision); and De Smet et al., 2019 (manuscript in preparation). A subset of the glioblastoma and oligodendroglioma spheroid models are currently characterized in the Cancer Dependency Map Project.

    Metastatic Brain Tumor Models

    Our metastatic brain tumor models have diverse origins of primary cancers (lung, skin, breast, colon, esophagus, testis, and others) – from the most common breast carcinoma and lung adenocarcinoma to the rare neuroendocrine carcinoma, prostate adenocarcinoma, yolk sac tumor, and small cell lung carcinoma. We recently presented this rare collection of models at the 2020 SNO Conference on Brain Metastases, and a recorded webinar is available.

  • Organ Site Diagnosis # of Models
    Brain Lymphoma CNS Secondary Diffuse Large B-Cell Lymphoma 6
    Brain Lymphoma CNS Secondary T-Cell Lymphoma 1
    Brain Metastasis Malignant Melanoma 15
    Brain Metastasis Breast Adenocarcinoma, ER+ 1
    Brain Metastasis Breast Adenocarcinoma, ER+ HER2+ 1
    Brain Metastasis Breast Adenocarcinoma, HER2+ 1
    Brain Metastasis Breast Carcinoma, ER+ 3
    Brain Metastasis Breast Carcinoma, ER+ HER2+ 5
    Brain Metastasis Breast Carcinoma, HER2+ 1
    Brain Metastasis Breast Carcinoma, Triple Negative 3
    Brain Metastasis Breast Ductal Adenocarcinoma, Triple Negative 1
    Brain Metastasis Breast Mucinous Adenocarcinoma, ER+ HER2+ 1
    Brain Metastasis Breast Squamous Cell Carcinoma, Triple Negative 1
    Brain Metastasis Colon Adenocarcinoma 5
    Brain Metastasis Colon Neuroendocrine Carcinoma 1
    Brain Metastasis Esophagus Adenocarcinoma 4
    Brain Metastasis Esophagus Carcinoma 1
    Brain Metastasis Larynx 1
    Brain Metastasis Lung Adenocarcinoma 15
    Brain Metastasis Lung Carcinoma 5
    Brain Metastasis Lung Mixed Cell Adenocarcinoma 1
    Brain Metastasis Lung Small Cell Carcinoma 5
    Brain Metastasis Ovary Serous Carcinoma 2
    Brain Metastasis Pancreas Adenocarcinoma 1
    Brain Metastasis Prostate Adenocarcinoma 1
    Brain Metastasis Sarcoma 6
    Brain Metastasis Uterine 1
    Brain Metastasis Yolk Sac Tumor 1


  • A collection of our 3D spheroid models are also luciferized and amendable for in vivo intracranial studies with bioluminescence imaging. Please see the table below.

    Luciferized Model ID Patient Methylation Status Diagnosis
    BT112 Unmethylated Glioblastoma NOS 4
    BT116 Unmethylated Glioblastoma NOS 4
    BT131 Unmethylated Glioblastoma NOS 4
    BT139 Methylated Glioblastoma NOS 4
    BT145 Not Profiled Glioblastoma NOS 4
    BT147 Unmethylated Glioblastoma NOS 4
    BT159 Unmethylated Glioblastoma NOS 4
    BT172 Unmethylated Glioblastoma NOS 4
    BT179 Not Profiled Glioblastoma IDH wildtype 4
    BT181 Methylated Glioblastoma NOS 4
    BT182 Unmethylated Glioblastoma NOS 4
    BT187 Not Profiled Glioblastoma NOS 4
    BT189 Not Profiled Glioblastoma NOS 4
    BT216 Unmethylated Glioblastoma NOS 4
    BT224 Methylated Glioblastoma NOS 4
    BT237 Not Profiled Anaplastic Oligodendroglioma IDH mutant and 1p 19q codeleted 3
    BT240 Unknown Glioblastoma NOS 4
    BT245 Not Profiled Glioblastoma NOS 4
    BT248 Unmethylated Glioblastoma NOS 4
    BT260 Not Profiled Anaplastic Oligodendroglioma NOS 3
    BT286 Methylated Glioblastoma NOS 4
    BT295 Unmethylated Glioblastoma NOS 4
    BT320 Unmethylated Glioblastoma IDH wildtype 4
    BT328 Methylated Glioblastoma IDH wildtype 4
    BT359 Unmethylated Glioblastoma IDH wildtype 4
    BT397 Methylated Glioblastoma IDH wildtype 4
    BT403 Unmethylated Glioblastoma IDH wildtype 4
    BT99 Unknown Glioblastoma NOS 4