CPDM's Collection of Primary and Metastatic Brain Tumor PDX and Spheroid, Organoid Models
CPDM has a large collection of primary and metastatic brain tumor models (230+ PDCLs and 90+ PDXs).
See our detailed model lists for more information about our brain tumor models. RNA-seq data, whole-exome seq data, and low-pass WGS data are available on cBioPortal. Genomic and transcriptomic data for these models is also available.
Learn more about ordering and licensing these models, or email us at models@dfci.harvard.edu to submit an inquiry.
See CPDM's SOPs for working with these models for guidance.
Primary Brain Tumor Models
Our primary brain tumor models cover various gliomas and include several rare IDH1 R132H models, as well as rare models of oligodendroglioma, medulloblastoma, and pineoblastoma, atypical teratoid/rhabdoid tumor.
| Organ Site | Diagnosis | # of Models |
|---|---|---|
| Brain | Glioblastoma IDH Wildtype 4 | 111 |
| Brain | Glioblastoma NOS 4 | 33 |
| Brain | Glioblastoma IDH Mutant 4 | 11 |
| Brain | Anaplastic Oligodendroglioma IDH Mutant And 1p 19q Codeleted 3 | 3 |
| Brain | Medulloblastoma 4 | 6 |
| Brain | Anaplastic Astrocytoma IDH Mutant 3 | 3 |
| Brain | High Grade Glioma NOS | 4 |
| Brain | Anaplastic Oligoastrocytoma 3 | 4 |
| Brain | Anaplastic Oligodendroglioma NOS 3 | 3 |
| Brain | Meningioma Atypical 2 | 3 |
| Brain | Pineoblastoma 4 | 2 |
| Brain | Meningioma Anaplastic 3 | 2 |
| Brain | Atypical Teratoid Rhabdoid Tumor 4 | 1 |
| Brain | Diffuse Astrocytoma IDH Mutant 2 | 1 |
Many of the brain tumor models have been previously published in various studies including Cancer Cell 2011; Nature 2012; Br J Cancer 2014; Oncotarget 2015; Cell Rep. 2016; Cell 2016; Clin Cancer Res. 2016; Nat Biotechnol. 2016; Cell Rep. 2017; PNAS 2017; Cancer Res. 2018; Genome Biol. 2018; Nature Commun. 2019; Nature 2020; Ferrer-Luna et al., 2019 (in revision); and De Smet et al., 2019 (manuscript in preparation); Cell Report 2021. A subset of the glioblastoma and oligodendroglioma spheroid models are currently characterized in the Cancer Dependency Map Project.
Metastatic Brain Tumor Models
Our metastatic brain tumor models have diverse origins of primary cancers (lung, skin, breast, colon, esophagus, testis, and others) – from the most common breast carcinoma and lung adenocarcinoma to the rare neuroendocrine carcinoma, prostate adenocarcinoma, yolk sac tumor, and small cell lung carcinoma. We recently presented this rare collection of models at the 2020 SNO Conference on Brain Metastases, and a recorded webinar is available.
| Organ Site | Diagnosis | # of Models |
|---|---|---|
| Brain | Lymphoma CNS Secondary Diffuse Large B-Cell Lymphoma | 6 |
| Brain | Lymphoma CNS Secondary T-Cell Lymphoma | 1 |
| Brain | Metastasis Malignant Melanoma | 15 |
| Brain | Metastasis Breast Adenocarcinoma, ER+ | 1 |
| Brain | Metastasis Breast Adenocarcinoma, ER+ HER2+ | 1 |
| Brain | Metastasis Breast Adenocarcinoma, HER2+ | 1 |
| Brain | Metastasis Breast Carcinoma, ER+ | 3 |
| Brain | Metastasis Breast Carcinoma, ER+ HER2+ | 5 |
| Brain | Metastasis Breast Carcinoma, HER2+ | 1 |
| Brain | Metastasis Breast Carcinoma, Triple Negative | 3 |
| Brain | Metastasis Breast Ductal Adenocarcinoma, Triple Negative | 1 |
| Brain | Metastasis Breast Mucinous Adenocarcinoma, ER+ HER2+ | 1 |
| Brain | Metastasis Breast Squamous Cell Carcinoma, Triple Negative | 1 |
| Brain | Metastasis Colon Adenocarcinoma | 5 |
| Brain | Metastasis Colon Neuroendocrine Carcinoma | 1 |
| Brain | Metastasis Esophagus Adenocarcinoma | 4 |
| Brain | Metastasis Esophagus Carcinoma | 1 |
| Brain | Metastasis Larynx | 1 |
| Brain | Metastasis Lung Adenocarcinoma | 15 |
| Brain | Metastasis Lung Carcinoma | 5 |
| Brain | Metastasis Lung Mixed Cell Adenocarcinoma | 1 |
| Brain | Metastasis Lung Small Cell Carcinoma | 5 |
| Brain | Metastasis Ovary Serous Carcinoma | 2 |
| Brain | Metastasis Pancreas Adenocarcinoma | 1 |
| Brain | Metastasis Prostate Adenocarcinoma | 1 |
| Brain | Metastasis Sarcoma | 6 |
| Brain | Metastasis Uterine | 1 |
| Brain | Metastasis Yolk Sac Tumor | 1 |
A collection of our 3D spheroid models are also luciferased and amendable for in vivo intracranial studies with bioluminescence imaging. Please see the table below.
| Luciferased Model ID | Patient Methylation Status | Diagnosis |
|---|---|---|
| BT112 | Unmethylated | Glioblastoma NOS 4 |
| BT116 | Unmethylated | Glioblastoma NOS 4 |
| BT131 | Unmethylated | Glioblastoma NOS 4 |
| BT139 | Methylated | Glioblastoma NOS 4 |
| BT145 | Not Profiled | Glioblastoma NOS 4 |
| BT147 | Unmethylated | Glioblastoma NOS 4 |
| BT159 | Unmethylated | Glioblastoma NOS 4 |
| BT172 | Unmethylated | Glioblastoma NOS 4 |
| BT179 | Not Profiled | Glioblastoma IDH wildtype 4 |
| BT181 | Methylated | Glioblastoma NOS 4 |
| BT182 | Unmethylated | Glioblastoma NOS 4 |
| BT187 | Not Profiled | Glioblastoma NOS 4 |
| BT189 | Not Profiled | Glioblastoma NOS 4 |
| BT216 | Unmethylated | Glioblastoma NOS 4 |
| BT224 | Methylated | Glioblastoma NOS 4 |
| BT237 | Not Profiled | Anaplastic Oligodendroglioma IDH mutant and 1p 19q codeleted 3 |
| BT240 | Unknown | Glioblastoma NOS 4 |
| BT245 | Not Profiled | Glioblastoma NOS 4 |
| BT248 | Unmethylated | Glioblastoma NOS 4 |
| BT260 | Not Profiled | Anaplastic Oligodendroglioma NOS 3 |
| BT286 | Methylated | Glioblastoma NOS 4 |
| BT295 | Unmethylated | Glioblastoma NOS 4 |
| BT320 | Unmethylated | Glioblastoma IDH wildtype 4 |
| BT328 | Methylated | Glioblastoma IDH wildtype 4 |
| BT359 | Unmethylated | Glioblastoma IDH wildtype 4 |
| BT397 | Methylated | Glioblastoma IDH wildtype 4 |
| BT403 | Unmethylated | Glioblastoma IDH wildtype 4 |
| BT99 | Unknown | Glioblastoma NOS 4 |