How We Diagnose Ovarian Cancer

Testing for Ovarian Cancer

Ovarian cancer is not considered just one type of cancer. There are several subtypes of ovarian cancer, and identifying those types requires careful evaluation by highly specialized pathologists. Making the correct diagnosis is key to tailoring treatment to your specific type of cancer.

Our pathology team is one of the few globally recognized groups that focuses entirely on gynecologic cancers, with thousands of diagnoses each year. Our internationally recognized gynecology specialists guide research teams across the country on how ovarian tumors develop and how they can be treated and prevented.

Our pathologists and researchers are skilled at diagnosing both common and rare ovarian cancers, including:

  • Epithelial: This is the most common type of ovarian cancer, which starts in the epithelial tissue, the lining on the outside of the ovary or in the fallopian tube. Epithelial ovarian cancer also includes primary peritoneal cancer and fallopian tube cancer. This type of cancer is divided into serous (high grade and low grade), mucinous, endometrioid (high grade and low grade), clear cell, transitional, and undifferentiated types. Ovarian carcinosarcoma is another rare type of epithelial ovarian cancer that we treat.
  • Germ cell: Germ cell tumors begin in the egg-producing cells. The main subtypes are teratoma, dysgerminoma, endodermal sinus tumor, and choriocarcinoma. We work closely with our pediatric oncology colleagues at Boston Children’s Hospital to devise specific and personalized treatment plans for patients with germ cell tumors.
  • Sex cord stromal: These rare tumors grow in the connective tissue that holds the ovary together and makes estrogen and progesterone. Sub-types include granulosa, granulosa-theca, and Sertoli-Leydig cell tumors.

Tests to Diagnose Ovarian Cancer

After our doctors carefully review your medical history and your family history of cancer, assess your physical exam results, and review radiographic imaging, they may recommend a biopsy either through radiology or surgery. A pathologic diagnosis will need to be made. This can occur through:

  1. Interventional radiology-guided biopsy
  2. Undergoing surgery in an operating room – either a biopsy or surgery to remove the cancer depending on the extent of the cancer  

The results from these tests are reviewed regularly by a dedicated team of gynecologic surgeons, medical oncologists, and radiation oncologists at a weekly meeting called a tumor board. This team will determine the best course of treatment specific to you and your type of ovarian cancer.

There is no standard screening test to identify ovarian cancer. Diagnosis of ovarian cancer begins with a pelvic exam with your doctor, which can be helpful in identifying a mass on either side of the uterus. 

Recommended Tests to Determine the Presence of Ovarian Cancer

  • Transvaginal ultrasound: A procedure in which high-energy sound waves (ultrasound) are bounced off internal tissues or organs, such as the vagina, uterus, fallopian tubes, and bladder. The wave echoes form a picture of body tissues called a sonogram.
  • Blood test: A test to measure a substance in the blood called CA-125 (a tumor marker that is often found to be elevated in the blood of women with ovarian cancer). This test is used to monitor the progress of treatment.
  • CT scan, PET/CT, and/or MRI scan and surgery: When the presence of ovarian cancer is detected, a CT scan or another radiologic procedure is performed to determine the extent of the disease. If the disease appears resectable (able to be removed through surgery), surgery is performed to make a definitive diagnosis and remove the tumor. If the disease does not appear to be resectable, a biopsy is performed to make a definitive diagnosis and determine the course of treatment.

Center for BRCA and Related Genes

Mutations in BRCA1, BRCA2, and BRCA-related genes confer increased risk of certain cancers when inherited. These mutations can also be acquired by the cancers themselves. The Mellen and Eisenson Family Center for BRCA and Related Genes provides comprehensive care for patients with inherited and acquired mutations, including clinical therapeutic trials, trials of risk-reducing strategies, and studies of novel early detection markers.

Inherited Gene Mutations

Up to 22 percent of ovarian, fallopian tube, and peritoneal cancers are caused by inherited gene mutations, such as the BRCA1 and BRCA2 genes and Lynch syndrome genes. Genetic testing is done to detect these mutated genes that can indicate a high risk of ovarian cancer.

Carrying a mutated gene does not mean that you will develop cancer or that cancer is currently present. Learning whether you carry a genetic mutation may influence how, how often, and when you and your family begin cancer screenings. In some cases, in patients who do not wish to have children or have completed their family, removing the fallopian tubes and ovaries will significantly reduce the risk of eventually developing ovarian or fallopian tube cancer. In these cases, the tubes and ovaries are carefully examined by pathologists who are experts in detecting early cancers.

If you or your doctor thinks you are at high risk of developing ovarian cancer, the Division of Cancer Genetics and Prevention at Dana-Farber can create a personalized program to accurately estimate your risk and work with you — together with your physicians and nurse practitioners — to lower your risk as much as possible.

Stages of Ovarian Cancer

Information from exams and diagnostic tests is used to determine the extent of the tumor and whether the cancer has spread to the lymph nodes or other tissues. The staging of ovarian cancer is the most important factor in determining a treatment plan.

The stages of epithelial ovarian cancer are:

  • Stage I indicates cancer in one or both ovaries. Cancer cells may be found on the surface of the ovaries, inside the ovaries, or in fluid surrounding the abdomen.
  • Stage II means that cancer is found in one or both ovaries, as well as other areas in the pelvis. Cancer cells can be found in the fallopian tubes, uterus, or other tissues in the pelvis, as well as the tissue or fluid surrounding the abdomen.
  • Stage III signifies cancer has spread beyond the ovaries and pelvis to the abdomen (abdominal wall or small intestines) or nearby lymph nodes. Cancer cells may also be found on the outside of the liver.
  • Stage IV indicates that cancer has spread beyond the ovaries, abdomen, and pelvis to other parts of the body, such as the lungs, the liver, or other tissues. Cancer cells found in the fluid around the lungs are also considered stage IV ovarian cancer.

Second Opinions

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Consultations and Second Opinions

Our diagnostic team provides second opinions, including for challenging or difficult cases. We are happy to consult with you, your primary care physician, or other specialists.

You may want to consider a second opinion:

  • To confirm your diagnosis
  • For an evaluation of an uncommon cancer
  • For details on the type and stage of cancer
  • To better understand your treatment options
  • To learn if you are eligible for clinical trials

Phone: 877-442-3324
Online: Complete our appointment request form

Can't travel to Boston? Our online second opinion service lets patients from all over the world receive expert second opinions from Dana-Farber oncologists.

For Referring Physicians

Because primary care physicians and community specialists are integral parts of every patient's care team, we are committed to collaborating with you in the care of your patient.

If you are a referring physician and have a patient with ovarian cancer, we look forward to working with you.

Find out more about how to refer a patient to Dana-Farber Brigham Cancer Center.