- Tool will be used to study accumulation of toxic protein in brain cells of patients with forms of dementia
- Represents first successful technique for targeted clearing of abnormal tau protein from human neurons
Harnessing a probe used to image the brain in diagnosing Alzheimer’s disease and related forms of dementia, scientists at Dana-Farber Cancer Institute and Massachusetts General Hospital (MGH) have successfully
cleared patient-derived brain cells of an abnormal protein associated with dementia and other neurodegenerative disorders.
The findings, reported in the journal eLife, provide a new tool to study the accumulation of abnormal tau protein that occurs in the brain cells of patients with Alzheimer’s and other forms of dementia, researchers say. It can help examine the
effects of the rapid removal of abnormal tau from cells and may even suggest ways of preventing or reversing a build-up of the protein.
“Tau has an important role in the cell: it binds to and stabilizes structures called microtubules, which give cells their shape,” says Fleur Ferguson, PhD, of Dana-Farber, co-first
author of the study with M. Catarina Silva, PhD, of MGH. “However, in neurodegenerative diseases such as Alzheimer’s, frontotemporal dementia [FTD], and progressive supranuclear palsy, abnormal tau accumulates in brain cells, which may be linked to
the progression of the disease. We want to understand more about the role these aberrant proteins play in disease.”
The new tool was created by taking a molecular probe used in positron emission tomography (PET) scanning for Alzheimer’s or other tau-related diseases and modifying it for a new purpose. The probe, called T807, binds specifically to the abnormal tau
protein. When the probe is tagged with a radioactive isotope, its binding to abnormal tau can be detected by a PET scanner, indicating the presence of disease.
To equip T807 for its new use, researchers left off the radioactive label and instead linked the compound to a molecule called an E3-ligase recruiting ligand, which binds to the cell’s machinery for destroying unwanted proteins. The new, dual-action form
of T807 – called QC-01-175 – grabs onto both abnormal tau and the protein-degradation machinery, bringing them close together. This proximity enables the abnormal tau to be ubiquitinated – bound to ubiquitin proteins – a modification that essentially
labels as cellular trash. Ubiquitination sends the unwanted tau to the cell’s proteasome, where it’s broken down and eliminated. In effect, QC-01-175 becomes a device for feeding toxic tau into the cell’s trash-disposal system.
When researchers tested the new tool for tau degradation in laboratory cultures of neurons derived from patients with tau mutations linked to FTD and cells from healthy volunteers, it worked as designed. It cleared away the abnormal tau from cells of
patients with FTD while having minimal effect on tau in neurons from healthy volunteers. (It did show some off-target effects, interfering with a small number of proteins other than abnormal tau, but researchers have strategies for reducing such effects.)
Importantly, treating FTD cells with QC-01-175 reduced their vulnerability to stress and improved their survival, indicating that degradation of abnormal tau may have therapeutic benefits.
The results demonstrate QC-01-175’s value as a research tool, the paper’s authors say, useful for exploring the process of abnormal tau accumulation and the effects on cells of rapid removal of the protein. They also highlight how targeted protein degradation
technology can be used to transform a protein binder into a functional molecule with pharmacological effects on cells. Researchers are now exploring this strategy in animal models of dementia and expanding the work to target additional proteins that
“This innovative strategy of controlling protein proximity to enable targeted protein degradation represents a powerful approach to potentially develop therapeutics for what have been otherwise intractable diseases of the brain,” says Stephen J. Haggarty,
PhD, of MGH Department of Neurology, the co-senior author of the paper with Nathanael Gray, PhD, of Dana-Farber. “We are tremendously excited about the potential for further translation
of these tools to enable the development of novel therapeutics for a wide range of neurological diseases.”
The co-authors of the study are: Quan Cai, PhD, Katherine A. Donovan, PhD, Tinghu Zhang, PhD, Hai-Tsang Huang, PhD, and Eric S. Fischer, PhD, of Dana-Farber; Ghata Nandi, MSc, Debasis Patnaik, PhD, Diane E. Lucente, MS, and Bradford C. Dickerson, MD,
of MGH; and Timothy J. Mitchison, PhD, of Harvard Medical School. This work was generously supported by the Rainwater Charitable Foundation/Tau Consortium and MGH Research Scholars Program.